Analysis of the transcription factor network during the early stage of iPSC production
| Project/Area Number |
25460354
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
Hisatake Koji 筑波大学, 医学医療系, 教授 (70271236)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA KEN 筑波大学, 医学医療系, 助教 (80500610)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 転写因子 / 遺伝子発現制御 / リプログラミング / iPS細胞 / 間葉上皮転換 / 遺伝子発現 / クロマチン / iPS 細胞 / エピジェネティクス / 細胞初期化 / センダイウイルス |
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| Outline of Final Research Achievements |
Genome-wide expression analyses were used to identify transcription factors whose expression decrease at the early stage or reprogramming. They were further narrowed down by other data base to select for the genes that are likely to be regulated directly by Oct4 and Klf4. The selection resulted in 38 candidate genes whose expression changes are potentially important for the early stage of reprogramming. siRNA-mediated knockdown and over-expression of these genes showed that down-regulation of Osr1、Ebf1、Ebf3、Meox2、Smardc3 and Prrx is essential at the early stage of reprogramming. The involvement of these genes in EMT or MET was examined by the used of NMuMG cells, and the Ebf3 was found to have an activity in promoting EMT.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] A Safeguard System for Induced Pluripotent Stem Cell Derived Rejuvenated T Cell Therapy2015
Author(s)
Ando M, Nishimura T, Yamazaki S, Yamaguchi T, Kawana-Tachikawa A, Hayama T, Nakauchi Y, Ando J, Ota Y, Takahashi S, Nishimura K, Ohtaka M, Nakanishi M, Miles JJ, Burrows SR, Brenner MK, Nakauchi H.
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Journal Title
Stem Cell Repots
Volume: 5(4)
Pages: 597-608
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Association of the winged helix motif of the TFIIEα subunit of TFIIE with either the TFIIEβ subunit or TFIIB distinguishes its functions in transcription2015
Author(s)
Tanaka, A., Akimoto, Y., Kobayashi, S., Hisatake, K., Hanaoka, F., and Ohkuma, Y.
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Journal Title
Genes to Cells
Volume: 20
Pages: 203-216
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Interspecific in vitro assay for chimera-forming ability of human pluripotent stem cells.2015
Author(s)
Masaki H, Kato-Itoh M, Umino A, Sato H, Hamanaka S, Kobayashi T, Yamaguchi T, Nishimura K, Ohtaka M, Nakanishi M, Nakauchi H.
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Journal Title
Development
Volume: 142(18)
Pages: 3222-30
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] The Paired-box Domain Transcription Factor Pax6 Binds to the Upstream Region of the TRAP Gene Promoter and Suppresses RANKL-induced Osteoclast Differentiation.2013
Author(s)
Kogawa M, Hisatake K, Atkins GJ, Findlay DM, Enoki Y, Sato T, Gray PC, Kanesaki-Yatsuka Y, Anerson PH, Wada S, Kato N, Fukuda A, Katayama S, Tsujimoto M, Yoda T, Suda T, Okazaki Y, Matsumoto M
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Journal Title
J. Biol. Chem.
Volume: 288
Pages: 31299-312
DOI
Related Report
Peer Reviewed
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