| Project/Area Number |
25460358
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Yamaguchi University (2015)
Tokyo Medical and Dental University (2013-2014) |
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Principal Investigator |
ASAOKA Yoichi 山口大学, 医学(系)研究科(研究院), 講師 (10436644)
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| Co-Investigator(Renkei-kenkyūsha) |
NISHINA Hiroshi 東京医科歯科大学, 難治疾患研究所, 教授 (60212122)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | Hippoシグナル伝達系 / YAP変異メダカ / 細胞張力 / 蛍光イメージング / 神経上皮組織 / FRET / 細胞内張力 / 網膜 |
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| Outline of Final Research Achievements |
Previously our group reported a unique medaka mutant, hirame (hir), which means flatfish in Japanese. Due to mutation of the yes-associated protein (YAP) transcriptional co-activator, hir embryos are sensitive to deformation by gravity and exhibit a markedly flattened body. In this study, we found that actomyosin-mediated tissue tension and fibronectin fibril formation are abnormal in hir embryos, leading to tissue flattening and tissue misalignment, both of which contribute to flattened body. YAP-knockdown human 3D spheroid also exhibits collapse upon exposure to external forces, which is reminiscent of the hir phenotype. Together, these findings reveal a novel mechanism of gravitational resistance in the 3D morphogenesis of a variety of animals including fish and humans.
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