The role of Hippo signaling pathway in the construction of three-dimensional epithelial tissues
Project/Area Number |
25460358
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General medical chemistry
|
Research Institution | Yamaguchi University (2015) Tokyo Medical and Dental University (2013-2014) |
Principal Investigator |
ASAOKA Yoichi 山口大学, 医学(系)研究科(研究院), 講師 (10436644)
|
Co-Investigator(Renkei-kenkyūsha) |
NISHINA Hiroshi 東京医科歯科大学, 難治疾患研究所, 教授 (60212122)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
Keywords | Hippoシグナル伝達系 / YAP変異メダカ / 細胞張力 / 蛍光イメージング / 神経上皮組織 / FRET / 細胞内張力 / 網膜 |
Outline of Final Research Achievements |
Previously our group reported a unique medaka mutant, hirame (hir), which means flatfish in Japanese. Due to mutation of the yes-associated protein (YAP) transcriptional co-activator, hir embryos are sensitive to deformation by gravity and exhibit a markedly flattened body. In this study, we found that actomyosin-mediated tissue tension and fibronectin fibril formation are abnormal in hir embryos, leading to tissue flattening and tissue misalignment, both of which contribute to flattened body. YAP-knockdown human 3D spheroid also exhibits collapse upon exposure to external forces, which is reminiscent of the hir phenotype. Together, these findings reveal a novel mechanism of gravitational resistance in the 3D morphogenesis of a variety of animals including fish and humans.
|
Report
(4 results)
Research Products
(30 results)
-
-
-
-
-
-
-
-
-
-
[Journal Article] Extracellular acidification activates ovarian cancer G-protein-coupled receptor 1 and GPR4 homologs of zebra fish.2015
Author(s)
Y. Mochimaru, M. Azuma, N. Oshima, Y. Ichijo, K. Satou, K. Matsuda, Y. Asaoka, H. Nishina, T. Nakakura, C. Mogi, K. Sato, F. Okajima, H. Tomura
-
Journal Title
Biochem. Biophys. Res. Commun.
Volume: 457
Issue: 4
Pages: 493-499
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-