| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We have found that anillin acts as an effector of IQGAP3 and mediates the function of IQGAP3 to regulate the process of mammalian cell cytokinesis. Anillin was found to directly interact with IQGAP3 and co-localize with IQGAP3 at the cleavage furrow in mitotic cells. It was shown that IQGAP3 is responsible for mammalian cell cytokinesis because of its ability to regulate the localization of anillin at the cleavage furrow. In addition, we have found that IQGAP1, another member of IQGAP family proteins, is also responsible for cytokinesis in mammalian cells, but it did so utilizing a molecular mechanisms distinct from that of IQGAP3. We have established IQGAP1- and IQGAP3-null cultured cells and are analyzing their phenotype in detail. Also, we have found a novel binding partner of IQGAP3 and are analyzing its molecular function.
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