| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The Ces1 gene encodes a Zinc finger-type transcription factor. Its expression is high in mouse embryonic stem (ES) cells, but rapidly decreases following differentiation. To assess its roles in ES cells, we generated Ces1-deficient and -overexpressing ES cell lines. We found that Ces1-deficient ES cells grow as flat colonies, which are clearly distinct from rounded colonies commonly formed by undifferentiated ES cells. The differentiation potency of Ces1-deficient ES cells was not considerably different from wild-type ES cells in vitro and in vivo. DNA microarray analysis revealed that the Ces1 knockout downregulated a distinct group of genes, including Dppa3. To identify the CES1-binding loci in the genome, chromatin immunoprecipitation sequencing was performed. The result showed that CES1 binding was often seen in the genes activated by Ces1 deficiency, suggesting the repressive role of Ces1.
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