| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The transcription factors T and Mesp1 play key roles in guiding embryonic stem cells (ESCs) toward mesodermal and cardiac lineages, respectively. Although a growing body of evidence indicates that T and Mesp1 regulate downstream target genes, the molecular mechanisms behind maintenance of their silencing state in the undifferentiated ESCs remain poorly understood.
Here we show that CIBZ, a BTB domain zinc finger transcriptional factor, is a novel negative regulator of mesodermal and cardiac differentiation of ESCs. We also show the direct inhibitory binding of CIBZ to T and Mesp1 promoters. These data provide new insights into the role of CIBZ in the regulation of cardiac specification of ESCs and show that CIBZ is critical for maintaining the silenced state of T and Mesp1 in ESCs.
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