| Project/Area Number |
25460371
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Kochi University |
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Principal Investigator |
Shuji Sakamoto 高知大学, 教育研究部医療学系基礎医学部門, 准教授 (80397546)
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| Research Collaborator |
Todaka Hiroshi 高知大学, 教育研究部医療学系基礎医学部門, 助教 (80769662)
Higuchi Takuma 高知大学, 教育研究部医療学系基礎医学部門, 助教 (10754567)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 二本鎖RNA結合蛋白質 / microRNA生合成 / miRNA / 筋分化 / 中心核病 / Dynamin 2 / NF90 / NF45 / 筋再生 / 中心核 / 骨格筋 / 筋成熟 / マイクロRNA / Dynamin / 筋萎縮 / ミオパチー / Dynamin2 |
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| Outline of Final Research Achievements |
1. The nuclear factor 90 (NF90) or the complex of NF90 with NF45 is known to be involved in microRNA biogenesis, mRNA stabilization, translational repression, replication of viral RNA and transcription in vitro. In this study, we found that the NF90-NF45 complex induces centronuclear myopathy through increased expression of dynamin 2, a causative gene of the disease, by an NF90-NF45-induced reduction of miR-133a expression in vivo.
2. We observed that the NF90-overexpressing HEK293 cells did not exhibit longer-term survival than the control cells transfected with mock plasmids when glucose was withdrawn, suggesting that cellular energy balance was not influenced by overexpression of NF90 in cultured cells in vitro.
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