Characterization and application for regenerative medicine of bone marrow pluripotent stem cells

• Project/Area Number: 25460373
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: National Institute of Infectious Diseases (2014-2015); Keio University (2013)
• Principal Investigator: Matsuoka Sahoko 国立感染症研究所, その他部局等, その他 (20317340)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 間葉性幹細胞 / 間葉系幹細胞

Outline of Final Research Achievements

We isolated SSEA-1dim Sca-1neg N-Cadherinpos undifferentiated cell population derived from endosteal mesenchymal cells in mouse bone marrow by a fluorescence activated cell sorter using pluripotent stem cell markers. Comprehensive gene expression analysis demonstrated that these cells show ES cell-like gene expression patterns. These cells were not increased under ES cell culture condition ande seemed to be domant in steady state. We examine the cell proliferation and differentiation under stress such as irradiation or drugs.