Search for the intracellular substrates and activators of coagulation factor XIII (plasma transglutaminase)
Project/Area Number |
25460379
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pathological medical chemistry
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Research Institution | Yamagata University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 酵素 / トランスグルタミナーゼ / 凝固第XIII因子 / 細胞内基質 / 細胞内活性化 / タンパク質架橋結合 / 巨核球 / 単球 / 細胞核 / 分化・成熟 |
Outline of Final Research Achievements |
The A subunit of coagulation factor XIII (FXIII-A) is a plasma transglutanimase (TGase), which exists not only in plasma but also inside bone marrow-derived cells such as megakaryocytes and monocytes/macrophages. In the present study, we examined the intracellular activation and activity of FXIII-A. We found that there was an activation factor(s) of FXIII-A in nucleus in the absence of proteolytic cleavage of the activation peptide in FXIII-A, and that at least three nuclear proteins were identified as possible substrates for FXIII-A. Furthermore, our study suggested that amine-modification of proteins by TGase activity regulates functions of the substrate proteins by the acceleration of proteasomal degradation in the absence of ubiquitination.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Clot retraction is mediated by factor XIII-dependent fibrin-aIIbb3-myosin axis in platelet sphingomyelin-rich membrane rafts2013
Author(s)
Kasahara, K., Kaneda, M., Miki, T., Iida, K., Sekino-Suzuki, N., Kawashima, I., Suzuki, H., Shimonaka, M., Arai, M., Ohni-Iwashita, Y., Kojima, S., Abe, M., Kobayashi, T., Okazaki, T., Souri, M., Ichinose, A., Yamamoto, N.
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Journal Title
Blood
Volume: 122(19)
Issue: 19
Pages: 3340-3348
DOI
Related Report
Peer Reviewed
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