Role of the regulation of B cell protein-tyrosine kinase by hepatitis C virus
| Project/Area Number |
25460383
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | University of Fukui |
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Principal Investigator |
SADA Kiyonao 福井大学, 医学部, 教授 (10273765)
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| Co-Investigator(Kenkyū-buntansha) |
CHIHARA Kazuyasu 福井大学, 医学部, 准教授 (00314948)
TAKEUCHI Kenji 福井大学, 医学部, 助教(学内講師) (40236419)
YAMAUCHI Shota 福井大学, 医学部, 特命助教 (00728941)
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| Co-Investigator(Renkei-kenkyūsha) |
HOTTA Hak 神戸大学, 保健学研究科, 教授 (40116249)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | C型肝炎ウイルス / チロシンキナーゼ |
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| Outline of Final Research Achievements |
Hepatitis C virus (HCV) infects B lymphocytes and induces B cell lymphoma, in addition to chronic hepatitis and hepatocellular carcinoma. However, molecular mechanism for the pathogenesis of HCV infection-mediated disorders remains unclear. In this study, we investigated the role of protein-tyrosine kinases on HCV lifecycle, because HCV interacts with protein-tyrosine kinases in B cells. As a result, we found that HCV particle assembly involves phosphorylation of NS5A (non-structural protein of HCV) by the c-Abl protein-tyrosine kinase.
In parallel with this study, we investigated the molecular mechanism of infection immunity. We identified the novel mast cell anti-fungal immunity through Dectin-1 and Syk, and novel regulatory mechanism of Syk substrate 3BP2 (c-Abl SH3 domain-binding protein-2) in B cells.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Hepatitis C virus particle assembly involves phosphorylation of NS5A by the c-Abl tyrosine kinase2015
Author(s)
Yamauchi, S., Takeuchi, K., Chihara, K., Sun, X., Honjoh, C., Yoshiki, H., Hotta, H., and Sada, K.
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Journal Title
J. Biol. Chem.
Volume: 290
Issue: 36
Pages: 21857-21864
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Tyrosine phosphorylation of 3BP2 is indispensable for the interaction with Vav3 in chicken DT40 cells2014
Author(s)
Chihara, K., Kimura, Y., Honjoh, C., Yamauchi, S., Takeuchi, K., Sada, K.
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Journal Title
Exp. Cell Res.
Volume: 322
Issue: 1
Pages: 99-107
DOI
Related Report
Peer Reviewed
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[Journal Article] Syk inhibitors2013
Author(s)
千原一泰、木村幸弘、本定千知、竹内健司、定 清直
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Journal Title
Japanese Journal of Clinical Immunology
Volume: 36
Issue: 4
Pages: 197-202
DOI
NAID
ISSN
0911-4300, 1349-7413
Related Report
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[Presentation] チロシンキナーゼによるHCV複製の制御機構2015
Author(s)
山内翔太, 竹内健司, 千原一泰, 孫雪東, 本定千知, 吉木はつみ, 堀田博, 定清直
Organizer
日本生化学会北陸支部第33回大会, シンポジウム「病原性ウイルス・免疫応答研究の最前線」
Place of Presentation
富山
Year and Date
2015-05-23
Related Report
Invited
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