| Project/Area Number |
25460396
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Suzuka University of Medical Science |
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Principal Investigator |
Suzuki Koji 鈴鹿医療科学大学, 薬学部, 教授 (70077808)
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| Co-Investigator(Renkei-kenkyūsha) |
HAYASHI Tatsuya 三重県立看護大学, 看護学部, 教授 (00242959)
OKAMOTO Takayuki 三重大学, 医学(系)研究科, 助教 (30378286)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | プロテインC凝固制御系 / 抗凝固因子 / 腫瘍促進作用 / プロテインCインヒビター / 抗腫瘍作用 / 活性化プロテインC / トロンボモジュリン / プロテインC / 凝固制御因子 / β1-3グルカン / 自然免疫 / 腫瘍細胞 / 増殖 / 転移 / ヘパリン / プロテインＣ / プロテインＣインヒビター |
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| Outline of Final Research Achievements |
Protein C inhibitor (PCI), a member of the serine protease inhibitors, physiologically inhibits an anticoagulant serine protease, activated protein C (APC) and thrombin- thrombomodulin (TM) complex. In this study, we studied the effects of host PCI on growth and metastasis of B16 melanoma (B16) cells by comparing between wild-type mice and mice transgenic for human PCI gene (hPCI-TG). B16 cells growth and metastatic nodules of B16 cells in the lungs were developed in hPCI-TG mice, and fibrin deposition in the lung in hPCI-TG mice was increased more than in wild-type mice. The invasive behavior of B16 cells observed in hPCI-TG mice was reduced by anti-human PCI IgG, APC, or soluble TM administration. Present studies suggest that PCI stimulates tumor cell growth and metastasis via its procoagulant properties, and anticoagulant proteins regulate PCI-dependent tumor cell growth and metastasis.
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