| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The aim of this study is to identify the gastric cancer stem cell. We found that KIFC1, KIF11, and IQGAP3 were expressed more than two-fold higher in spheroid body-forming cells than in parental cells by microarray analysis. Both the number and size of spheres from gastric cancer cells were significantly reduced upon KIFC1, KIF11, and IQGAP3 siRNA-transfection compared with negative control siRNA-transfection. Immunohistochemical analysis demonstrated that these molecules were overexpressed in gastric cancer. Gastric cancer cases positive for KIFC1 were found more frequently in intestinal type cases. Gastric cancer cases positive for KIF11 were found more frequently in intestinal mucin cases, and those positive for IQGAP3 were found more frequently in gastric mucin cases. In esophageal squamous cell carcinoma, podoplanin was expressed in chemo-radiation therapy-resistant cells. Our results showed that KIFC1, KIF11, and IQGAP3 likely plays an important role in cancer stem cell.
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