Identification of gastric cancer stem cell and its association with gastric/intestinal mucin phenotype
Project/Area Number |
25460417
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Human pathology
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Research Institution | Hiroshima University |
Principal Investigator |
Oue Naohide 広島大学, 医歯薬保健学研究院(医), 准教授 (60346484)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 病理学 / 胃癌 / 癌幹細胞 / KIF / IQGAP3 / 幹細胞 / KIFC1 / KIF11 / 癌 |
Outline of Final Research Achievements |
The aim of this study is to identify the gastric cancer stem cell. We found that KIFC1, KIF11, and IQGAP3 were expressed more than two-fold higher in spheroid body-forming cells than in parental cells by microarray analysis. Both the number and size of spheres from gastric cancer cells were significantly reduced upon KIFC1, KIF11, and IQGAP3 siRNA-transfection compared with negative control siRNA-transfection. Immunohistochemical analysis demonstrated that these molecules were overexpressed in gastric cancer. Gastric cancer cases positive for KIFC1 were found more frequently in intestinal type cases. Gastric cancer cases positive for KIF11 were found more frequently in intestinal mucin cases, and those positive for IQGAP3 were found more frequently in gastric mucin cases. In esophageal squamous cell carcinoma, podoplanin was expressed in chemo-radiation therapy-resistant cells. Our results showed that KIFC1, KIF11, and IQGAP3 likely plays an important role in cancer stem cell.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Overexpression of Transmembrane Protein BST2 is Associated with Poor Survival of Patients with Esophageal, Gastric, or Colorectal Cancer.2016
Author(s)
Mukai S, Oue N, Oshima T, Mukai R, Tatsumoto Y, Sakamoto N, Sentani K, Tanabe K, Egi H, Hinoi T, Ohdan H, Yasui W
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Journal Title
Ann Surg Oncol
Volume: 印刷中
Pages: 印刷中
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Fukutin, identified by the Escherichia coli ampicillin secretion trap (CAST) method, participates in tumor progression in gastric cancer.2016
Author(s)
Oo HZ, Sentani K, Mukai S, Hattori T, Shinmei S, Goto K, Sakamoto N, Naito Y, Anami K, Trang PT, Yanagihara K, Oue N, Yasui W
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Journal Title
Gastric Cancer
Volume: 19
Pages: 443-52
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] TSPAN8, identified by Escherichia coli ampicillin secretion trap, is associated with cell growth and invasion in gastric cancer.2016
Author(s)
Anami K, Oue N, Noguchi T, Sakamoto N, Sentani K, Hayashi T, Naito Y, Oo HZ, Yasui W
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Journal Title
Gastric Cancer
Volume: 19
Pages: 370-80
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Characteristic miR-24 Expression in Gastric Cancers among Atomic Bomb Survivors.2015
Author(s)
Naito Y, Oue N, Pham TT, Yamamoto M, Fujihara M, Ishida T, Mukai S, Sentani K, Sakamoto N, Hida E, Sasaki H, Yasui W
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Journal Title
Pathobiology
Volume: 82
Pages: 68-75
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] TSPAN8, identified by Escherichia coli ampicillin secretion trap, is associated with cell growth and invasion in gastric cancer.2015
Author(s)
Anami K, Oue N, Noguchi T, Sakamoto N, Sentani K, Hayashi T, Naito Y, Oo HZ, Yasui W
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Journal Title
Gastric Cancer
Volume: 印刷中
Pages: 印刷中
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] New molecular staging with G-factor supplements TNM classification in gastric cancer: a multicenter collaborative research by the Japan Society for Gastroenterological Carcinogenesis G-Project committee.2015
Author(s)
Sawada T, Yashiro M, Sentani K, Oue N, Yasui W, Miyazaki K, Kai K, Fushida S, Fujimura T, Ohira M, Kakeji Y, Natsugoe S, Shirabe K, Nomura S, Shimada Y, Tomita N, Hirakawa K, Maehara Y
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Journal Title
Gastric Cancer
Volume: 18
Pages: 119-128
DOI
Related Report
Peer Reviewed
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[Journal Article] MicroRNA-145 is a potential prognostic factor of scirrhous type gastric cancer.2014
Author(s)
Naito Y, Yasuno K, Tagawa H, Sakamoto N, Oue N, Yashiro M, Sentani K, Goto K, Shinmei S, Oo HZ, Yanagihara K, Hirakawa K, Yasui W
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Journal Title
Oncol Rep
Volume: 32
Pages: 1720-1726
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Identification of PRL1 as a novel diagnostic and therapeutic target for castration-resistant prostate cancer by the Escherichia coli ampicillin secretion trap (CAST) method.2014
Author(s)
Shinmei S, Sentani K, Hayashi T, Sakamoto N, Goto K, Zarni Oo H, Naito Y, Teishima J, Matsubara A, Oue N, Kuniyasu H, Yasui W
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Journal Title
Urol Oncol
Volume: 32
Pages: 769-778
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] MicroRNA-148a is downregulated in gastric cancer, targets MMP7, and indicates tumor invasiveness and poor prognosis.2014
Author(s)
Sakamoto N, Naito Y, Oue N, Sentani K, Uraoka N, Zarni Oo H, Yanagihara K, Aoyagi K, Sasaki H, Yasui W
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Journal Title
Cancer Sci
Volume: 105
Pages: 236-243
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] High miR-21 expression from FFPE tissues is associated with poor survival and response to adjuvant chemotherapy in colon cancer.2014
Author(s)
Oue N, Anami K, Schetter AJ, Moehler M, Okayama H, Khan MA, Bowman ED, Mueller A, Schad A, Shimomura M, Hinoi T, Aoyagi K, Sasaki H, Okajima M, Ohdan H, Galle PR, Yasui W, Harris CC
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Journal Title
Int J Cancer
Volume: 134
Pages: 1926-1934
DOI
Related Report
Peer Reviewed
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[Journal Article] Signal peptidase complex 18, encoded by SEC11A, contributes to progression via TGF-α secretion in gastric cancer.2014
Author(s)
Oue N, Naito Y, Hayashi T, Takigahira M, Kawano-Nagatsuma A, Sentani K, Sakamoto N, Zarni Oo H, Uraoka N, Yanagihara K, Ochiai A, Sasaki H, Yasui W
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Journal Title
Oncogene
Volume: 33
Pages: 3918-3926
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Canonical Wnt signals combined with suppressed TGFβ/BMP pathways promote renewal of the native human colonic epithelium.2014
Author(s)
Reynolds A, Wharton N, Parris A, Mitchell E, Sobolewski A, Kam C, Bigwood L, El Hadi A, Münsterberg A, Lewis M, Speakman C, Stebbings W, Wharton R, Sargen K, Tighe R, Jamieson C, Hernon J, Kapur S, Oue N, Yasui W, Williams MR
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Journal Title
Gut
Volume: 63
Pages: 610-621
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] SEC11A contributes to malignant progression through promotion of EGF and TGF-alpha secretion.2013
Author(s)
Oue N, Hayashi T, Takigahara M, Nagatsuma A, Sentani K, Sakamoto N, Anami K, Uraoka N, Naito Y, Yanagihara K, Ochiai A, Sasaki H and Yasui W
Organizer
The 104th Annual Meeting of the American Association for Cancer Research
Place of Presentation
Washington D.C., Maryland (USA)
Year and Date
20130406-20130410
Related Report
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