| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In cell culture, by adding MMP7 and TGF-beta1, human cultured fibroblasts and umbilical vein endothelial cells were altered. In particular, the endothelial-endothelial junction decreased by MMP7. Therefore, it was suggested that cancer stroma around MMP7-producing tumor cells influenced the infiltration of tumor cell into vessels and promotes vascular invasion.
We performed immunohistochemical staining of MSR1, a marker of macrophages associated with MMP7-related angiogenesis, for colorectal carcinoma with early hematogenous metastasis. The expression of MSR1 was observed in macrophages around cancer cells at invasive front of carcinoma. MMP7 expression of cancer cells was observed mainly in invasive front. Therefore, it was suggested that MMP7-positive cancer cells invade in vessels in association with the presence of macrophages.
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