DNA methylation alterations in autoimmune pancreatitis

• Project/Area Number: 25460453
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Shinshu University
• Principal Investigator: UEHARA Takeshi 信州大学, 学術研究院医学系, 准教授 (80402121)
• Co-Investigator(Kenkyū-buntansha): MASUMOTO Junya 愛媛大学, 大学院学医学系研究科ゲノム病理学分野, 教授 (20334914)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 自己免疫性膵炎 / メチル化解析 / メチル化異常

Outline of Final Research Achievements

We used an Illumina Infinium MethylationEPIC Kit to conduct a comprehensive analysis of DNA methylation in tissues of patients with autoimmune pancreatitis (AIP). Controls included pancreatic tissue acquired from patients with obstructive pancreatitis caused by pancreatic carcinomas as well as from normal pancreatic tissue. We compared genes with promoter hypermethylation between patients with AIP and the controls. The validation of the method was affected by technical problems. Immunohistochemical analysis will be required to determine if promoter hypermethylation of specific genes is involved in the pathogenesis of AIP.

Research Products

• [Journal Article] Multifocal Fibrosclerosis with Hypertrophic Pachymeningitis and a Soft Tissue Mass around the Thoracic Vertebral Bodies: A Case Report with Review of the Literature (2015)
  • Author(s): Sato M, Kodaira M, Ishii W, Nakagawa M, Kishida D, Miyazaki D, Shimojima Y, Matsuda M, Uehara T, Ikeda S.
  • Journal Title: Internal Medicine Volume: 54 Issue: 17 Pages: 2267-2272
  • DOI: 10.2169/internalmedicine.54.3230
  • NAID: 130005097140
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed
• [Journal Article] Signal intensity of the pancreas on magnetic resonance imaging: Prediction of postoperative pancreatic fistula after a distal pancreatectomy using a triple-row stapler (2015)
  • Author(s): Arai T, Kobayashi A, Yokoyama T, Ohya A, Fujinaga Y, Shimizu A, Motoyama H, Furusawa N, Sakai H, Uehara T, Kadoya M, Miyagawa S.
  • Journal Title: Pancreatology Volume: 15 Issue: 4 Pages: 380-386
  • DOI: 10.1016/j.pan.2015.05.479
  • Peer Reviewed
• [Journal Article] IgG4関連疾患と前立腺疾患 (2015)
  • Author(s): 上原剛、小川輝之、黒住昌弘
  • Journal Title: 腎臓内科・泌尿器科 Volume: 2 Pages: 313-319
  • NAID: 40022582519
• [Journal Article] 免疫炎症検査と新しい疾患概念 IgG4関連疾患の病態と検査 自己免疫性膵炎を中心に(解説) (2015)
  • Author(s): 上原剛
  • Journal Title: 臨床病理 Volume: 63 Pages: 1202-1206
• [Journal Article] 電気泳動検査が発見の端緒となった自己免疫性膵炎の診断基準 (2015)
  • Author(s): 菅野光俊、上原剛、石嶺南生、宇佐美陽子、川崎健治、濱野英明、本田孝行
  • Journal Title: 電気泳動 Volume: 59 Pages: 35-38
  • NAID: 130005131088
• [Journal Article] 【胆道癌として外科切除された鑑別困難病変の検証-画像と病理所見の対比-】 胆管癌とIgG4関連硬化性胆管炎との術前鑑別診断の現状 (2014)
  • Author(s): 村木 崇, 渡邉 貴之, 濱野 英明, 川 茂幸, 上原 剛, 藤永 康成, 越知 泰英, 梶川 昌二, 丸山 真弘, 金井 圭太, 小口 貴也, 浅野 純平, 新倉 則和
  • Journal Title: 胆と膵 Volume: 35 Pages: 233-240
• [Journal Article] Inflammation of colon adenoma in the setting of type 1 autoimmune pancreatitis (2014)
  • Author(s): Uehara T, Hamano H, Suga T, Kawa S, Yoshizawa A, Kobayashi Y, Murata K, Oki K, Sano K, Onodera R, Ota H.
  • Journal Title: Pathology international Volume: 64 Pages: 67-74
  • Peer Reviewed
• [Journal Article] Comparison of histopathological features of pancreatic carcinoma and type 1 autoimmune pancreatitis (2014)
  • Author(s): Uehara T, Hamano H, Kawa S, Kobayashi Y, Yoshizawa A, Oki K, Nakata R, Kobayashi A, Sano K, Ota H.
  • Journal Title: Pathology international Volume: 64 Pages: 51-57
  • Peer Reviewed
• [Journal Article] IgG4-related disease-like fibrosis as an indicator of IgG4-related lymphadenopathy (2013)
  • Author(s): Uehara T, Masumoto J, Yoshizawa A, Kobayashi Y, Hamano H, Kawa S, Oki K, Oikawa N, Honda T, Ota H.
  • Journal Title: Annals of Diagnostic Pathology Volume: 17 Pages: 416-420
  • Peer Reviewed
• [Presentation] IgG4関連眼疾患診断基準による当科症例の検討 (2016)
  • Author(s): 黒川徹、村田敏規、浜野英明、藤永康成、上原剛、杠俊介、茂木英明
  • Organizer: 第9回 IgG4研究会
  • Place of Presentation: ピュアリティまきび （ 岡山県岡山市 北区下石井２丁目６－４１）
  • Year and Date: 2016-03-19
• [Presentation] IgG4関連膵胆管病変における内視鏡の役割 IgG4関連硬化性胆管炎に対する経乳頭的胆管生検の有用性と限界 (2015)
  • Author(s): 伊藤哲也、木村崇、上原 剛
  • Organizer: 第89回日本消化器内視鏡学会総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市熱田区熱田西町1番1号）
  • Year and Date: 2015-05-31
• [Presentation] 肺結節に対して肺切除術を施行されたIgG4関連疾患の臨床画像病理学的検討 (2015)
  • Author(s): 野沢修平、山本 洋、立石一成、 牛木淳人、漆畑一寿、安尾将法、 花岡正幸、吉田和夫、 川上聡、 吉澤明彦、 上原剛
  • Organizer: 第55回日本呼吸器学会学術講演会
  • Place of Presentation: 東京国際フォーラム（東京都千代田区丸の内３丁目５－１）
  • Year and Date: 2015-04-18
• [Presentation] 免疫炎症検査と新しい疾患概念 IgG4関連疾患の病態と検査 (2015)
  • Author(s): 上原剛
  • Organizer: 第１０回日本臨床検査医学会特別例会
  • Place of Presentation: グランドプリンスホテル京都（京都）
  • Year and Date: 2015-04-13
  • Invited
• [Presentation] 術前診断が困難であった限局性の自己免疫性膵炎の一例 (2014)
  • Author(s): 神宮 邦彦, 上原 剛, 古澤 徳彦, 横山 隆秀, 宮川 眞一
  • Organizer: 第103回 日本病理学会
  • Place of Presentation: 広島国際会議場（広島市）
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] 膵癌と 1 型自己免疫性膵炎の組織学的 (2014)
  • Author(s): 上原剛，浜野英明，川茂幸，吉澤明彦，佐野健司，小林幸弘，仲田梨恵，小林聡，浅香志穂，立石文子，大月聡明，神宮邦彦，的場久典，太田浩良
  • Organizer: 第103回 日本病理学会 総会
  • Place of Presentation: 広島国際会議場（広島県広島市）
• [Book] 臨床検査法提要、14病理検査 (2015)
  • Author(s): 上原剛、太田浩良、小林幸弘
  • Publisher: 金原出版株式会社
• [Book] Molecular Pathology of Neoplastic Gastrointestinal Diseases, 10. Molecular Pathology of Inflammatory Bowel Disease Associated Neoplasia (2013)
  • Author(s): Takeshi Uehara, Deqin Ma, and Antonia R. Sepulveda
  • Total Pages: 6
  • Publisher: Springer