Study of a novel therapy of the triple-negative breast cancers (TNBC) -based on DNA copy number aberration.

• Project/Area Number: 25460458
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Yamaguchi University
• Principal Investigator: KONOD-FURUYA Tomoko 山口大学, 医学(系)研究科(研究院), 講師 (30379979)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: トリプルネガティブ乳癌 / ゲノム異常 / イメージサイトメトリー / 細胞周期 / 乳癌 / トリプルネガティブ / 分子標的薬

Outline of Final Research Achievements

Triple-negative breast cancer (TNBC) is refractory breast cancer because specific therapies are not establieshed. We focused on interferon gamma (IFN-gamma) which is located in the genomic loss region exclusively in TNBC and investigated the effect of INF-gamma on TNBC in vitro. Several cell lines derived from TNBC were inhibited cell proliferation by IFN-gamma. However, one cell line from TNBC was not inhibited. INF-gamma altered the cell cycle and the expression levels of some cell-cylce associated proteins in TNBC cell lines. The pattens of change in the expression leves of theses proteins were different in cell lines. This suggest that IFN-gamma arrest cell cycles via several pathway in TNBC.

Research Products

• [Journal Article] がん細胞のタンパク質情報を考慮に入れた、SVMによる病理診断システムの可能性 (2015)
  • Author(s): 北風裕教、重本昌也、古屋智子、松野浩嗣
  • Journal Title: 産業応用工学会論文誌 Volume: 3 Pages: 56-64
  • NAID: 130005263502
  • Peer Reviewed
• [Journal Article] Analysis of centromere signal patterns in breast cancer cells with chromosomal instability using image cytometry combined with centromere fluorescence in situ hybridization. (2014)
  • Author(s): Ito H, Oga A, Ikemoto K, Furuya T, Maeda N, Yamamoto S, Kawauchi S, Itoh H, Oka M, Sasaki K.
  • Journal Title: Cytometry A Volume: 85 Issue: 9 Pages: 809-816
  • DOI: 10.1002/cyto.a.22502
  • Peer Reviewed
• [Journal Article] DN多型 (2013)
  • Author(s): 末広 寛、古屋 智子、佐々木 功典、日野田 裕治
  • Journal Title: 日本臨床検査医学会誌 Volume: 61 Pages: 1001-1007
  • Peer Reviewed
• [Journal Article] Quantitative analysis of centrromeric FISH spots during the cell cycle by image cytometry (2013)
  • Author(s): Amakawa G, Ikemoto K, Ito H, Furuya T, Sasaki K
  • Journal Title: J Histochem Cytochem Volume: 61 Issue: 10 Pages: 699-705
  • DOI: 10.1369/0022155413498754
  • Peer Reviewed
• [Journal Article] Elucidation of proliferative capability of mononuclear tetraploid cells, emerging spontaneously from diploid cells, using image cytometry and fluorescence in situ hybridization. (2013)
  • Author(s): Ito H, Oga A, Furuya T, Ikemoto K, Amakawa G, Chochi Y, Kawauchi S, Sasaki K
  • Journal Title: Cell Prolif Volume: 46(3) Issue: 3 Pages: 356-363
  • DOI: 10.1111/cpr.12032
  • Peer Reviewed
• [Journal Article] Germline copy number variations associated with breast cancer susceptibility in a Japanese population. (2013)
  • Author(s): Suehiro Y, Okada T, Shikamoto N, Zhan Y, Sakai K, Okayama N, Nishioka M, Furuya T, Oga A, Kawauchi S, Maeda N, Tamesa M, Nagashima Y, Yamamoto S, Oka M, Hinoda Y, Sasaki K
  • Journal Title: Tumour Biol Volume: 34(2) Issue: 2 Pages: 947-952
  • DOI: 10.1007/s13277-012-0630-x
  • Peer Reviewed
• [Presentation] 病理組織の分子遺伝学的解析によるゲノム変化と臨床病態との関連について (2016)
  • Author(s): 古屋智子
  • Organizer: 第105回日本病理学会
  • Place of Presentation: 仙台国際センター（宮城県・仙台市）
  • Year and Date: 2016-05-12
• [Presentation] イメージサイトメーターを用いたインターフェロンγの細胞増殖抑制効果に関する研究 (2015)
  • Author(s): 古屋智子、伊藤秀明、北風裕教、小賀厚徳、松野浩嗣、佐々木功典、伊藤浩史
  • Organizer: 日本サイトメトリー学術集会
  • Place of Presentation: ソラシティカンファレンスセンター（東京都千代田区）
  • Year and Date: 2015-07-11
• [Presentation] インターフェロンガンマの乳癌由来培養細胞への影響についての細胞周期的解析 (2015)
  • Author(s): 近藤（古屋）智子、原田美沙、伊藤秀明、帖地康代、小賀厚徳、河内茂人、佐々木功典、伊藤浩史
  • Organizer: 第104回 日本病理学会総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-04-30 – 2015-05-02
• [Presentation] イメージサイトメトリーを用いた染色体不安定性の解析 (2013)
  • Author(s): 伊藤 秀明、小賀 厚徳、古屋 智子、池本 健三、帖地 康世、佐々木 功典、河内 茂人
  • Organizer: 日本病理学会総会
  • Place of Presentation: ロイトン札幌 札幌芸文館 （札幌市）
• [Presentation] ヒト骨髄由来間葉系幹細胞の長期培養におけるゲノム変化ついての検討 (2013)
  • Author(s): 古屋 智子、伊藤 秀明、帖地 康世、池本 健三、小賀 厚徳、河内 茂人、佐々木 功典
  • Organizer: 日本病理学会総会
  • Place of Presentation: ロイトン札幌 札幌芸文館 （札幌市）