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Transplantation of human endothelial progenitor cells for the therapeutic approach to spinal cord ischemia reperfusion injury in mice

Research Project

Project/Area Number 25460477
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Experimental pathology
Research InstitutionKobe University

Principal Investigator

Okita Yutaka  神戸大学, 医学(系)研究科(研究院), 教授 (40322193)

Co-Investigator(Kenkyū-buntansha) Kawamoto Atsuhiko  公益財団法人先端医療振興財団, 先端医療センター 再生医療研究部 血管再生研究グループ, グループリーダー (00275330)
Fujita Yasuyuki  公益財団法人先端医療振興財団, 先端医療センター 再生医療研究部 血管再生研究グループ, 研究員 (00590465)
Asahara Takayuki  東海大学, 医学部, 教授 (20246200)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsマウス脊髄虚血再灌流障害 / ヒト血管内皮前駆細胞 / CD34陽性細胞 / 胸腹部大動脈手術合併症 / 対麻痺 / マウス脊髄虚血再灌流障害モデル / 胸腹部大動脈瘤合併症 / 胸腹部大動脈手術 / 脊髄虚血 / 血管内皮前駆細胞
Outline of Final Research Achievements

Spinal cord ischemia injury (SCI) remains one of devastating complications associated with surgery and endovascular repair for descending thoracic and thoracoabdominal aortic diseases. Recently, the efficacy and safety of human endothelial progenitor cell therapy for spinal cord injury as well as ischemic diseases have been revealed in preclinical and clinical studies. Mouse delayed and immediate-onset SCI models were established in C57BL/6 strain. However, only immediate-onset but not delayed-onset SCI was induced in severe combined immunodeficiency (SCID) mice. We investigated the efficacy of intravenous injection of human CD34+ cells at 8 hours after reperfusion using SCID mouse immediate-onset SCI model. Immuno-histochemically, transplanted cells were abundantly located extra and intra vasculum at ischemic site of spinal cord, however, viable motor neuron was not detected at 40 hours after cell. Therefore, no benefit of CD34+ cell therapy was detected in this severe SCI model.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2016 2015 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (3 results) Book (1 results)

  • [Journal Article] Impact of acquired and innate immunity on spinal cord ischemia and reperfusion injury2016

    • Author(s)
      Yamanaka K, Sasaki N, Fujita Y, Kawamoto A, Hirata K-i, Okita Y
    • Journal Title

      General Thoracic and Cardiovascular Surgery

      Volume: 64 Issue: 5 Pages: 251-259

    • DOI

      10.1007/s11748-016-0629-0

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ninjurin1 – A Novel Regulator of Angiogenesis Mediated by Pericytes –2015

    • Author(s)
      Fujita Y, Kawamoto A
    • Journal Title

      Circulation Journal

      Volume: 79 Issue: 6 Pages: 1218-1219

    • DOI

      10.1253/circj.CJ-15-0435

    • NAID

      130005073134

    • ISSN
      1346-9843, 1347-4820
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phase II Clinical Trial of CD34+ Cell Therapy to Explore Endpoint Selection and Timing in Patients With Critical Limb Ischemia2014

    • Author(s)
      Yasuyuki Fujita; Takayuki Asahara; Yutaka Okita; Atsuhiko Kawamoto et al.
    • Journal Title

      Circulation Journal

      Volume: 78 Issue: 2 Pages: 490-501

    • DOI

      10.1253/circj.CJ-13-0864

    • NAID

      130003382211

    • ISSN
      1346-9843, 1347-4820
    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cell-Based Therapies for Peripheral Arterial Disease2014

    • Author(s)
      Fujita Y, Kawamoto A
    • Journal Title

      J Stem Cell Res Ther

      Volume: 4 Issue: 09 Pages: 234-234

    • DOI

      10.4172/2157-7633.1000234

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] INTRACORONARY ADMINISTRATION OF GCSF-MOBILIZED HUMAN CD34+ CELLS PRESERVES LEFT VENTRCULAR FUNCTION AND PREVENTS INFARCT EXPANSION IN A SWINE MODEL OF ACUTE MYOCARDIAL INFARCTION2015

    • Author(s)
      Yasuyuki Fujita, Makoto Kinoshita, Hiroshi Akimaru, Erika Akimaru, Miki Komatsu, Yutaka Furukawa, Takayuki Asahara, Atsuhiko Kawamoto
    • Organizer
      iSSCR ANNUAL MEETING
    • Place of Presentation
      Stockholm, Sweden
    • Year and Date
      2015-06-24 – 2015-06-27
    • Related Report
      2014 Research-status Report
  • [Presentation] ブタ急性心筋梗塞モデルにおけるCD34陽性細胞の冠動脈内注入:左室機能および梗塞サイズに対する効果2015

    • Author(s)
      藤田靖之、木下 愼、秋丸裕司、秋丸恵理佳、小松美希、古川 裕、浅原孝之、川本篤彦
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜(会議センター)
    • Year and Date
      2015-03-18 – 2015-03-19
    • Related Report
      2014 Research-status Report
  • [Presentation] Intracoronary Infusion of GCSF-Mobilized Human CD34+ Cells Preserves Left Ventricular Function and Prevents Infarct Expansion in a Swine Model of Acute Myocardial Infarction.2014

    • Author(s)
      Yasuyuki Fujita, Makoto Kinoshita, Hiroshi Akimaru, Erika Akimaru, Miki Komatsu, Yutaka Furukawa, Takayuki Asahara, Atsuhiko Kawamoto
    • Organizer
      American Heart Association Scientific Sessions 2014.
    • Place of Presentation
      Chicago, Illinois
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2014 Research-status Report
  • [Book] Biochemical Basis and Therapeutic Implications of Angiogenesis, ‘Angiogenesis in Myocardial Ischemia’ Advances in Biochemistry in Health and Disease2013

    • Author(s)
      Yasuyuki Fujita, Takayuki Asahara, Atsuhiko kawamoto
    • Total Pages
      23
    • Publisher
      Springer
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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