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Molecular and functional analysis of YAP/TAZ protein complex

Research Project

Project/Area Number 25460482
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Experimental pathology
Research InstitutionAichi Medical University

Principal Investigator

Murakami Hideki  愛知医科大学, 医学部, 准教授 (90303619)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsがん遺伝子 / 転座 / YAP/TAZ / 類上皮血管内皮腫 / 類上皮血管肉腫 / TAZ / 質量分析解析
Outline of Final Research Achievements

The TAZ/CAMTA1 or YAP/TFE3 fusion genes have been identified in epithelioid hemangioendothelioma (EHE). However, the detailed function of TAZ/CAMTA1 and YAP/TFE3 on cell growth or motility remains unclear. To investigate the biological roles of these fusion proteins in EHE, we generated lentivirus mediated TAZ/CAMTA1 or YAP/TFE3 expression system in HMEC1 cells, and analyzed the changes in cell growth, motility, and the interacting proteins with fusion protein using a tandem affinity purification with LC-MS/MS. Fusion proteins were localized in the nucleus, whereas TAZ and YAP proteins preferentially found in the cytoplasm. Expression of TAZ/CAMTA modestly increased cell proliferation and motility. Using tandem affinity purification coupled with LC-MS/MS, we identified several TAZ/CAMTA-associated proteins, such as ERG, SIR7, AHNK. Our results will provide new insights into development of EHE, and give some clues to developing a new molecular target therapy for EHE.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2016 2015 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results)

  • [Journal Article] Meigs Syndrome Superimposed on Gorlin Syndrome in a 14-Year-Old Girl.2016

    • Author(s)
      Iwasaki K, Matsushita H, Murakami H, Watanabe K, Wakatsuki A
    • Journal Title

      J Pediatr Adolesc Gynecol

      Volume: 29 Issue: 5 Pages: 75-77

    • DOI

      10.1016/j.jpag.2016.03.010

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional differences between wild-type and mutant-type BRCA1-associated protein 1 tumor suppressor against malignant mesothelioma cells.2015

    • Author(s)
      Hakiri S, Osada H, Ishiguro F, Murakami H, Murakami-Tonami Y, Sekido Y.
    • Journal Title

      Cancer science

      Volume: 106 Issue: 8 Pages: 990-999

    • DOI

      10.1111/cas.12698

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] β-catenin mutation in ovarian solid pseudopapillary neoplasm2014

    • Author(s)
      Kominami A, Fujino M, Murakami H, Ito M
    • Journal Title

      Pathology international

      Volume: 64 Issue: 9 Pages: 460-464

    • DOI

      10.1111/pin.12194

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] GLI1 interferes with the DNA mismatch repair system in pancreatic cancer through BHLHE41-mediated suppression of MLH1.2013

    • Author(s)
      Ingaguma S, RIku M, Hashimoto M, Murakami H, Saga S, Ikeda H, Kasai K
    • Journal Title

      Cancer Research

      Volume: 73 Issue: 24 Pages: 7313-7323

    • DOI

      10.1158/0008-5472.can-13-2008

    • Related Report
      2013 Research-status Report
    • Peer Reviewed

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Published: 2014-07-25   Modified: 2019-07-29  

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