Investigation of c-ktlow murine hematopoietic stem cells for the role in aged animals

• Project/Area Number: 25460483
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kansai Medical University
• Principal Investigator: SASAKI Yutaka 関西医科大学, 医学部, 准教授 (80425066)
• Co-Investigator(Renkei-kenkyūsha): SONODA Yoshiaki 関西医科大学, 医学部, 教授 (60206688)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 造血幹細胞 / G0 / c-kit

Outline of Final Research Achievements

Hematopoietic stem cells (HSCs) stay long in G0 of cell cycle status. The c-kitlow subfraction of the Lin-Sca-1+c-kit+CD150+CD48-HSCs contains cells in G0 in high frequency. Observation in vivo of integrated BrdU in HSCs suggested that HSCs divided slowly with fluctuating c-kit expression in the time course. The c-kitlow subfraction included cells that did not divide in response to pro-proliferation stimuli. These cells were poor in mitochondrial mass and in activities of reactive oxygen species. DNA microarray analyses indicated that c-kitlow cells differed from c-kithigh cells in the level of cell cycle related gene expression as well as genes for transcription, phosphorylation and metabolism.

Research Products

• [Presentation] In vivo cell division kinetics for the cells of different c-kit intensity in murine HSC compartment (2015)
  • Author(s): 佐々木豊、松岡由和、中塚隆介、角出啓輔、河村孟、藤岡龍哉、薗田精昭
  • Organizer: 第77回日本血液学会学術集会
  • Place of Presentation: 石川県立音楽堂など（金沢市）
  • Year and Date: 2015-10-16
• [Presentation] FACS-Segregated C-Kithigh and C-Kitlow Cells In The Murine Hematopoietic Stem Cell Compartment Are Functionally Distinct (2015)
  • Author(s): Sasaki Y, Matsuoka Y, Nakatsuka R, Sumide K, Kawamura H, Fujioka T, Sonoda Y
  • Organizer: 44th Annual Scientific Meeting of the ISEH
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2015-09-17
  • Int'l Joint Research
• [Presentation] Investigation of the lower c-kit expressing cells in murine hematopoietic stem cell compartment (2015)
  • Author(s): Yutaka Sasaki, Yoshikazu Matsuoka, Ryusuke Nakatsuka, Keisuke Sumide, Hiroshi Kawamura, Tatsuya Fujioka, Yoshiaki Sonoda
  • Organizer: 第13回幹細胞シンポジウム
  • Place of Presentation: 東京大学伊藤国際学術センター（東京都文京区）
  • Year and Date: 2015-05-29 – 2015-05-30
• [Presentation] Investigation of the lower c-kit expressing cells in murine hematopoietic stem cell compartment (2015)
  • Author(s): Yutaka Sasaki, Yoshikazu Matsuoka, Ryusuke Nakatsuka, Keisuke Sumide, Hiroshi Kawamura, Tatsuya Fujioka, Yoshiaki Sonoda
  • Organizer: 13th Stem Cell Research Symposium
  • Place of Presentation: 東京大学 伊藤国際学術研究センター（文京区）
  • Year and Date: 2015-05-29
• [Presentation] Characterization of the lower c-kit expressing cells in murine hematopoietic stem cell compartment (2014)
  • Author(s): Yutaka Sasaki, Yoshikazu Matsuoka, Ryusuke Nakatsuka, Keisuke Sumide, Tatsuya Fujioka, Yoshiaki Sonoda
  • Organizer: 第76回日本血液学会学術集会
  • Place of Presentation: 大阪国際会議場（大阪市）
  • Year and Date: 2014-10-31 – 2014-11-02
• [Presentation] マウス骨髄造血幹細胞分画における低c-kit発現細胞の検討 (2014)
  • Author(s): 佐々木豊、松岡由和、中塚隆介、角出啓輔、藤岡龍哉、薗田精昭
  • Organizer: 第24回日本サイトメトリー学会学術集会
  • Place of Presentation: 関西医科大学（枚方市）
  • Year and Date: 2014-06-28 – 2014-06-29
• [Presentation] マウス骨髄造血幹細胞分画における低c-kit発現細胞の検討 (2014)
  • Author(s): 佐々木 豊、松岡由和、中塚隆介、角出啓輔、藤岡龍哉、薗田精昭
  • Organizer: 第24回日本サイトメトリー学会学術集会
  • Place of Presentation: 関西医科大学（大阪府枚方市）
• [Presentation] マウス骨髄造血幹細胞分画におけるc-kit発現レベルについての検討 (2013)
  • Author(s): 佐々木 豊、松岡由和、中塚隆介、角出啓輔、藤岡龍哉、薗田精昭
  • Organizer: 第23回日本サイトメトリー学会学術集会
  • Place of Presentation: 橘桜会館（東京都文京区）