Epigenome profiling of precancerous condition in hepatocarcinogenesis using purified human hepatocytes
Project/Area Number |
25460487
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Experimental pathology
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Research Institution | Keio University (2015) National Cancer Center Japan (2013-2014) |
Principal Investigator |
Arai Eri 慶應義塾大学, 医学部, 講師 (40446547)
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Co-Investigator(Renkei-kenkyūsha) |
GOTOH Masahiro 国立研究開発法人国立がん研究センター, 研究所, 主任研究員 (00291138)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | エピゲノム / 純化細胞 / 前がん状態 / 肝細胞がん / 慢性肝炎 / 肝硬変 / 肝炎ウイルス / DNAメチル化 / ヒストン修飾 / ウイルス性肝炎 / アルコール性肝炎 / 非アルコール性脂肪肝 |
Outline of Final Research Achievements |
Thirty-one samples of hepatocytes with precancerous and normal condition could be purified from partial hepatectomy specimens of patients with metastatic carcinoma and hepatocellular carcinoma associated with hepatitis B or C viral infection. Methylome analysis was performed using Infinium HumanMethylation450k BeadChip. The incidence of DNA methylation alterations was higher at the precancerous stage of HCV-positive patients than that of HBV-positive patients. Many Infinium probes showed HCV-positive-specific DNA methylation alterations throughout the genome, by contrast to HBV-positive patient-specific ones. Although DNA methylation profile of purified hepatocytes exhibited almost similar trend with liver tissue samples, some characteristic profiles appeared significant in purified cells. Cell purification from surgical specimen enabled histone modification profiling which is applicable to personal differentiation or clinical analysis.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Epigenetic clustering of gastric carcinomas based on DNA methylation profiles at the precancerous stage: its correlation with tumor aggressiveness and patient outcome.2015
Author(s)
Yamanoi K, Arai E, Tian Y, Takahashi Y, Miyata S, Sasaki H, Chiwaki F, Ichikawa H, Sakamoto H, Kushima R, Katai H, Yoshida T, Sakamoto M, Kanai Y.
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Journal Title
Carcinogenesis
Volume: 36
Issue: 5
Pages: 509-520
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Alterations of the spindle checkpoint pathway in clinicopathologically aggressive CpG island methylator phenotype clear cell renal cell carcinomas.2015
Author(s)
Arai E, Gotoh M, Tian Y, Sakamoto H, Ono M, Matsuda A, Takahashi Y, Miyata S, Totsuka H, Chiku S, Komiyama M, Fujimoto H, Matsumoto K, Yamada T, Yoshida T, Kanai Y.
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Journal Title
Int J Cancer
Volume: 137
Issue: 11
Pages: 2589-2606
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Silencing of microRNA-122 is an early event during hepatocarcinogenesis from non-alcoholic steatohepatitis.2014
Author(s)
Takaki Y, Saito Y, Takasugi A, Toshimitsu K, Yamada S, Muramatsu T, Kimura M, Sugiyama K, Suzuki H, Arai E, Ojima H, Kanai Y, Saito H.
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Journal Title
Cancer Sci.
Volume: 105
Issue: 10
Pages: 1254-1260
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Multilayer-omics analysis of renal cell carcinoma, including the whole exome, methylome and transcriptome.2014
Author(s)
Arai E, Sakamoto H, Ichikawa H, Totsuka H, Chiku S, Gotoh M, Mori T, Nakatani T, Ohnami S, Nakagawa T, Fujimoto H, Wang L, Aburatani H, Yoshida T, Kanai Y.
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Journal Title
Int J Cancer
Volume: 135
Issue: 6
Pages: 1330-1342
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Comprehensive exploration of novel chimeric transcripts in clear cell renal cell carcinomas using whole transcriptome analysis.2014
Author(s)
Gotoh M, Ichikawa H, Arai E, Chiku S, Sakamoto H, Fujimoto H, Hiramoto M, Nammo T, Yasuda K, Yoshida T, Kanai Y.
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Journal Title
Genes Chromosomes Cancer
Volume: 53
Issue: 12
Pages: 1018-1032
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Epigenome landscape of human normal purified hepatocytes and genome-epigenome interaction: analysis by the International Human Epigenome Consortium (IHEC)2016
Author(s)
Arai E, Miura F, Totoki Y, Yamashita S, Tian Y, Gotoh M, Ojima H, Nakamura H, Hama N, Kato M, Kimura H, Suzuki Y, Ito T, Shibata T, Kanai Y
Organizer
Tenth AACR-JCA Joint Conference on Breakthroughs in Cancer Research: From Biology to Therapeutics
Place of Presentation
マウイ(米国)
Year and Date
2016-02-16
Related Report
Int'l Joint Research
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[Presentation] Reference epigenome mapping of hepatocytes: participation in International Human Epigenome Consortium (IHEC)2015
Author(s)
Arai E, Miura F, Totoki Y, Yamashita S, Tian Y, Gotoh M, Ojima H, Nakamura H, Hama N, Suzuki Y, Ito T, Shibata T, Kanai Y
Organizer
第74回日本癌学会学術総会
Place of Presentation
名古屋国際会議場(愛知県名古屋市)
Year and Date
2015-10-08
Related Report
Int'l Joint Research
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[Presentation] Multilayer omics analyses in CpG island methylator phenotype clear cell renal cell carcinomas2014
Author(s)
Arai E, Sakamoto H, Ono M, Takahashi Y, Miyata S, Fujimoto H, Gotoh M, Yamada T, Kanai Y.
Organizer
American Association of Cancer Research 105th Annual Meeting 2014
Place of Presentation
San Diego, CA, USA
Year and Date
2014-04-05 – 2014-04-09
Related Report
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[Presentation] Multilayer-Omics (Whole-Exome, Methylome and Transcriptome) Analysis Identifies the Wnt/β-catenin Pathway as a Key Player in the Development of Renal Cell Carcinoma2013
Author(s)
Arai E, Sakamoto H, Ichikawa H, Totsuka H, Gotoh M, Mori T, Ohnami S, Nakagawa T, Fujimoto H, Wang L, Aburatani H, Yoshida T, Kanai Y
Organizer
103rd American Association of Cancer Research Annual Meeting 2013
Place of Presentation
Washington DC, USA
Related Report
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[Presentation] Epigenome mapping in purified human hepatocytes2013
Author(s)
Arai E, Miura F, Yamashita S, Shibata T, Ito T, Suzuki Y, Tian Y, Gotoh M, Ojima H, Kosuge T, Kanai Y
Organizer
IHEC Annual Meeting 2013, Berlin
Place of Presentation
Berlin, Germany
Related Report
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