| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Thirty-one samples of hepatocytes with precancerous and normal condition could be purified from partial hepatectomy specimens of patients with metastatic carcinoma and hepatocellular carcinoma associated with hepatitis B or C viral infection. Methylome analysis was performed using Infinium HumanMethylation450k BeadChip. The incidence of DNA methylation alterations was higher at the precancerous stage of HCV-positive patients than that of HBV-positive patients. Many Infinium probes showed HCV-positive-specific DNA methylation alterations throughout the genome, by contrast to HBV-positive patient-specific ones. Although DNA methylation profile of purified hepatocytes exhibited almost similar trend with liver tissue samples, some characteristic profiles appeared significant in purified cells. Cell purification from surgical specimen enabled histone modification profiling which is applicable to personal differentiation or clinical analysis.
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