| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The Sleeping Beauty transposon-induced glioblastoma model was successfully introduced in the Lgr5-eGFP transgenic mouse. Precise collections of the mouse glioblastoma tissues were feasible. Several lines of neurosphere culture using a neural stem cell culture system derived from the mouse glioblastoma tissues were established. Higher Lgr5 expression levels in the GFP positive fraction were confirmed. The observations that the Lgr5 positive cells exerted higher tumorigenicity in vivo indicated that the cancer stem cell properties were enriched in the Lgr5 positive glioblastoma cells. Global gene expression analysis was performed, which identified the shh-pathway as a signal activated in the Lgr5 positive population and higher expression of CDKNs in Lgr5 negative population. Furthermore, Gli2 was identified as an indispensible factor for the glioblastoma stem cells.
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