Characterization of Lgr5+ cells using a transposon-induced glioblastoma mouse model and identification of novel therapeutic targets
| Project/Area Number |
25460488
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Kyoto Pharmaceutical University (2014-2015)
愛知県がんセンター(研究所) (2013) |
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Principal Investigator |
Nakata Susumu 京都薬科大学, 薬学部, 准教授 (80590695)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Mitsugu 近畿大学医学部, 細菌学教室, 准教授 (40609997)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 癌幹細胞 / 脳腫瘍 / 膠芽腫 / Lgr5 / Wnt / Shh / がん幹細胞 / マウスモデル / LGR5 / マウス発癌モデル / 幹細胞マーカー |
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| Outline of Final Research Achievements |
The Sleeping Beauty transposon-induced glioblastoma model was successfully introduced in the Lgr5-eGFP transgenic mouse. Precise collections of the mouse glioblastoma tissues were feasible. Several lines of neurosphere culture using a neural stem cell culture system derived from the mouse glioblastoma tissues were established. Higher Lgr5 expression levels in the GFP positive fraction were confirmed. The observations that the Lgr5 positive cells exerted higher tumorigenicity in vivo indicated that the cancer stem cell properties were enriched in the Lgr5 positive glioblastoma cells. Global gene expression analysis was performed, which identified the shh-pathway as a signal activated in the Lgr5 positive population and higher expression of CDKNs in Lgr5 negative population. Furthermore, Gli2 was identified as an indispensible factor for the glioblastoma stem cells.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Polycomb group protein Ezh2 regulates hepatic progenitor cell proliferation and differentiation in murine embryonic liver.2014
Author(s)
Koike H, Ouchi R, Ueno Y, Nakata S, Obana Y, Sekine K, Zheng YW, Takebe T, Isono K, Koseki H, Taniguchi H.
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Journal Title
PLoS One.
Volume: 9
Issue: 8
Pages: 0-2
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Ring1B Promotes Hepatic Stem/Progenitor Cell Expansion via Simultaneous Suppression of Cdkn1a and Cdkn2a.2014
Author(s)
Koike H, Ueno Y, Naito T, Shiina T, Nakata S, Ouchi R, Obana Y, Sekine K, Zheng YW, Takebe T, Isono K, Koseki H, Taniguchi H
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Journal Title
Hepatology
Volume: 印刷中
Issue: 1
Pages: 323-333
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Analysis of chemoradiotherapy resistance in the human pancreatic cancer stem cells
Author(s)
星野早百合, 関根圭輔, 孫略, 中田晋, 寺崎哲也, 森永聡一郎, 宮城洋平, 遠藤格, 横瀬智之, 上野康晴, 倉田昌直, 谷口英樹
Organizer
第72回 日本癌学会学術総会
Place of Presentation
パシフィコ横浜(横浜)
Related Report
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