Functional in vivo cellular imaging of graft-versus-host disease in allogeneic hematopoietic stem cell transplantation in mice

Research Project

Project/Area Number	25460509
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Experimental pathology
Research Institution	National Cancer Center Japan
Principal Investigator	Ikarashi Yoshinori 国立研究開発法人国立がん研究センター, 研究所, 外来研究員 (70280782)
Project Period (FY)	2013-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	イメージング / 同種造血幹細胞移植 / 移植片対宿主病 / GFP / 蛍光イメージング / アポトーシス / 生体イメージング / 蛍光タンパク質 / 細胞周期
Outline of Final Research Achievements	Visualizing the function of donor cells after allogeneic hematopoietic stem cell transplantation (HSCT) will enable understanding of the process of graft-versus-host disease (GVHD). In this study, we visualized the proliferation and apoptosis of green fluorescent protein protein (GFP) donor cells using immunofluorescence staining and in vivo fluorescence imaging. Immunofluorescence staining revealed that a large fraction of donor cells were cell proliferation marker, Ki-67 positive and a few host but not donor mononuclear cells were apoptotic cell marker, cleaved Caspase-3 positive in the secondary lymphoid organ at early phase after HSCT. We could not visualize apoptotic cell using in vivo fluorescence imaging. However, it is very important for visualizing cellular proliferation and apoptosis in vivo to understand the process of GVHD.