Induction of ex vivo-pulsed self dendritic cell vaccine against congenital toxoplasmosis
Project/Area Number |
25460512
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Parasitology (including sanitary zoology)
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Research Institution | Shinshu University (2014-2015) Chiba University (2013) |
Principal Investigator |
AOSAI Fumie 信州大学, 医学部, 委嘱講師 (80150316)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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Keywords | 先天性トキソプラズマ症 / トキソプラズマHSP70 / 樹状細胞ワクチン / 細胞傷害性T細胞(CTL) / T細胞ペプチドエピトープ / 細胞傷害性T細胞 (CTL) / T細胞ペピチドエピトープ / 樹状細胞(DC)ワクチン / トキソプラズマHSP70 (T.g.HSP70) / 細胞傷害性T細胞(CTL) / Th1防御免疫 |
Outline of Final Research Achievements |
1.Toxoplasma gondii infection during pregnancy induced the intrauterine growth retardation in fetus. The influences of T. gondii infection on placenta and fetal liver were shown by analyzing their dendritic cell (DC) maturation and Th polarization. 2. Not only CD8+ but CD4+ cytotoxic T lymphocytes (CTL) specific for T. gondii-infected cells were induced by the vaccination with a virulent tachyzoite-specific HSP70 (T.g.HSP70) gene targeting peripheral DC. Adoptive transfer experiments revealed that the vaccine-induced CTL possessed protective roles for toxoplasmosis at both acute and chronic phases of infection. CTL peptide epitopes of T.g.HSP70 which bound to murine and human MHC molecules were analyzed.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Successful treatment of toxoplasmic encephalitis early diagnosed by a polymerase chain reaction after allogeneic hematopoietic stem cell transplantation: two cases reports and review of the literature.2015
Author(s)
Miyagi T, Itonaga H, Aosai F, Taguchi J, Norose K, Mochizuki K, Fujii H, Furumoto A, Ohama M, Karimata K, Yamanoha A, Taniguchi H, Sato S, Taira N, Moriuchi Y, Fukushima T, Masuzaki H, Miyazaki Y.
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Journal Title
Transplant Infect Dis.
Volume: 17
Issue: 4
Pages: 593-8
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Acute exacerbation of Toxoplasma gondii infection after hematopoietic stem cell transplantation: five case reports among 279 recipients2013
Author(s)
Sumi M, Aosai F, Norose K, Takeda W, Kirihara T, Sato K, Fujikawa Y, Shimizu I, Ueki T, Hirosima Y, Ueno M, Ichikawa N, Watanabe M, Kobayashi H.
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Journal Title
International Journal of Hematology
Volume: 98
Pages: 214-222
Related Report
Peer Reviewed
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