Development of comprehensive detection methods of protozoan parasites using molecular taxonomy
Project/Area Number |
25460514
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Parasitology (including sanitary zoology)
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Research Institution | Kanazawa University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | 分子分類 / 種内多型 / DNAバーコード / 原虫 / アメーバ属 / 鞭毛虫 / トリコモナス属 / トリパノソーマ / 腸管寄生原虫 / DNAバーコーディング / アメーバ / 赤痢アメーバ / ジアルジア / DNAバーゴーディング |
Outline of Final Research Achievements |
A systemic screening method targeting protozoan parasites was developed, and applied the methods to various clinical examinations and molecular epidemiologic investigations. The achieved results are; (1) Lower prevalence of Entamoeba species in children with vertically transmitted HIV infection were observed in Western Kenya. (2) The genetic identification of Entamoeba polecki was confirmed from pigs in Japan and revealed its potential pathogenic role in the ulcerative colitis. (3) Genetic identification of various trichomonads species isolated from humans and related mammals were confirmed. The data showed the wide distribution of an specific genotype of Pentatrichomonas hominis among human and animals, and thus P. hominis is considered to be a zoonotic protozoan. (4) Other achievements; Clinical diagnoses of giardiasis and amebic liver access. The molecular epidemiologic investigation of Trypanosoma sp. among Japanese wild rodents could reveal the presence of T. grosi.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Lower prevalence of Entamoeba species in children with vertically transmitted HIV infection in Western Kenya. AIDS.2016
Author(s)
Matey EJ, Tokoro M, Nagamoto T, Mizuno T, Saina MC, Bi X, Oyombra JA, Okumu P, Langat BK, Sang WK, Songok EM, Ichimura H.
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Journal Title
AIDS
Volume: 30
Issue: 5
Pages: 803-805
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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