| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
Merozoite invasion of malaria parasite is attributed with the parasite erythrocyte binding antigens (EBA). In this study, we attempted to screen for identification of the receptor molecules on the surface of the host RBC for the EBAs. By means of comprehensive immuno-precipitation with more than 200 polyclonal antibodies, we identified the molecule interacting with GPI-anchored micronemal antigen (GAMA), a blood stage vaccine candidate. We named the molecule GAMA association protein (GAAP). Biacore analysis revealed that Kd value of the interaction was 2.5 nM. We also identified the GAAP interacting domain in GAMA which has about 100 amino acid residues.
|
