Cellular and molecular mechanisms of protection against reinfection with Vampirolepis nana eggs and expulsion of adult worms

• Project/Area Number: 25460520
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Jikei University School of Medicine
• Principal Investigator: Watanabe Naohiro 東京慈恵会医科大学, 医学部, 教授 (00057019)
• Co-Investigator(Kenkyū-buntansha): 石渡 賢治 東京慈恵会医科大学, 医学部, 准教授 (00241307) ; 浅野 和仁 昭和大学, 保健医療学部, 教授 (80159376)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 小形条虫 / 感染防御 / 自然免疫 / Ｔ細胞 / ＩｇＥ / マスト細胞 / 獲得免疫 / 消化管免疫 / IgE / 腸管免疫

Outline of Final Research Achievements

This study examined the mechanisms of protection against reinfection with Vampirolepis nana eggs and expulsion of adult worms in mice. Protection against reinfection with eggs was strongly induced by innate immunity under co-existence of CD4-positive abT cells and gdT cells, and acquired immunity through CD4-positive abT cells. Expulsion of V.nana adults from the intestine after primary and secondary infection depended on antibody and mast cells with different mechanisms.

Research Products

• [Journal Article] Impaired protection against Trichinella spiralis in mice with high levels of IgE (2014)
  • Author(s): Watanabe N.
  • Journal Title: Parasitology International Volume: 63 Issue: 2 Pages: 332-336
  • DOI: 10.1016/j.parint.2013.12.004
  • Peer Reviewed
• [Journal Article] The skin is an important bulwark of acquired immunity against intestinal helminths (2013)
  • Author(s): Obata-Ninomiya, K., Ishiwata, K., Tsutsui, H., Nei, Y., Yoshikawa, S., Kawano, Y., Minegishi, Y., Ohta, N., Watanabe, N., Kanuka, H., and Karasuyama, H.
  • Journal Title: J. Exp. Med. Volume: 210 Issue: 12 Pages: 2583-2595
  • DOI: 10.1084/jem.20130761
  • Peer Reviewed
• [Presentation] Ｐｒｏｔｅｃｔｉｖｅ ｉｍｍｕｎｉｔｙ ａｇａｉｎｓｔ Ｖａｍｐｉｒｏｌｅｐｉｓ ｎａｎａ ａｄｕｌｔ ｗｏｒｍｓ (2017)
  • Author(s): Ｗａｔａｎａｂｅ Ｎ， Ｉｓｈｉｗａｔａ Ｋ， Ａｓａｎｏ Ｋ
  • Organizer: 第８６回日本寄生虫学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2017-05-28
• [Presentation] Ｅｘｐｕｌｓｉｏｎ ｏｆ Ｖａｍｐｉｒｏｌｅｐｉｓ ｎａｎａ ｆｒｏｍ ｔｈｅ ｉｎｔｅｓｔｉｎｅ ｉｎ ｍｉｃｅ (2016)
  • Author(s): Ｗａｔａｎａｂｅ Ｎ， Ｉｓｈｉｗａｔａ Ｋ， Ａｓａｎｏ Ｋ
  • Organizer: 第４５回日本免疫学会学術集会
  • Place of Presentation: 宜野湾市
  • Year and Date: 2016-12-05
• [Presentation] 小形条虫の感染における免疫防御機構 (2016)
  • Author(s): 渡邊直熙、石渡賢治、池田愛、浅野和仁
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2016-03-19
• [Presentation] Protection to reinfection with Vampirolepis nana eggs depending on innate and acquired immunity in mice. (2015)
  • Author(s): Watanabe N., Ishiwata K., Asano K.
  • Organizer: 第44回日本免疫学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2015-12-18
• [Presentation] 小形条虫の虫卵再感染に対する防御機構 (2015)
  • Author(s): 渡邊直熙、石渡賢治、浅野和仁
  • Organizer: 第26回日本生体防御学会総会
  • Place of Presentation: 東京
  • Year and Date: 2015-07-10
• [Presentation] 自然免疫と獲得免疫による小形条虫の虫卵再感染防御 (2015)
  • Author(s): 渡邊直熙、石渡賢治、浅野和仁
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-20 – 2015-03-21
• [Presentation] Protection to reinfection with Vampirolepis nana eggs depending on innate immunity in mice. (2014)
  • Author(s): Watanabe N., Ishiwata K., Asano K.
  • Organizer: 第43回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2014-12-11 – 2014-12-13
• [Presentation] 小形条虫の虫卵再感染における自然免疫による防御 (2014)
  • Author(s): 渡邊直熙、石渡賢治、浅野和仁
  • Organizer: 第25回日本生体防御学会学術総会
  • Place of Presentation: 仙台
  • Year and Date: 2014-07-10 – 2014-07-11
• [Presentation] 小形条虫の虫卵による再感染の防御機構 (2014)
  • Author(s): 渡邊直熙、石渡賢治、浅野和仁
  • Organizer: 第８３回日本寄生虫学会大会
  • Place of Presentation: 松山（愛媛大）
• [Presentation] Rapid induction of protection to reinfection with Vampirolepis nana eggs in mice. (2013)
  • Author(s): Watanabe N., Ishiwata K., Asano K.
  • Organizer: 日本免疫学会総会・学術集会 2013
  • Place of Presentation: 千葉県幕張メッセ
• [Book] 寄生虫学研究 材料と方法 ２０１３年版 (2013)
  • Author(s): 横山卓也、嘉糠洋陸、渡邊直熙、浅野和仁
  • Publisher: 三恵社