| Project/Area Number |
25460525
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Tomita Takashi 国立感染症研究所, 昆虫医科学部, 室長 (20180169)
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| Co-Investigator(Kenkyū-buntansha) |
駒形 修 国立感染症研究所, 昆虫医科学部, 研究員 (20435712)
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| Research Collaborator |
ITOKAWA Kentaro 国立感染症研究所, 昆虫医科学部, 流動研究員 (70769992)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 殺虫剤抵抗性 / シトクロムP450 / 遺伝子重複 / 遺伝子過剰発現 / シス作用性変異 / 遺伝子ノックアウト / ネッタイイエカ / 殺虫剤抵抗性機構 / ゲノム編集 / レポーターアッセイ / 転写調節 / TALEN / CRISPR/Cas9 / 過剰発現 / TALENs / CRSPR/Cas9 |
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| Outline of Final Research Achievements |
Genetic studies have revealed that pyrethroid resistance of Culex quinquefasciatus is strongly associated with overexpressing CYP9M10 haplotypes. In this study, we demonstrated that a point mutation in the core promotor region of CYP9M10 is attributable to the elevated transcription. Moreover, bioassays using CYP9M10-knocked out mosquitoes derived from a pyrethroid resistant strain proved the primary responsibility of CYP9M10 for metabolic resistance to permethrin in larval stages.
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