Elucidation of the mechanism of Cholix cytotoxicity via its receptor.
| Project/Area Number |
25460526
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Chiba University |
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Principal Investigator |
Yahiro Kinnosuke 千葉大学, 医学(系)研究科(研究院), 准教授 (80345024)
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| Co-Investigator(Renkei-kenkyūsha) |
OGURA Kohei 国立国際医療センター研究所, 博士研究員 (00586612)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 毒素 / サイトカイン / 受容体 / 細胞死 / エフェクター / 細菌 / ADP-リボシル化 / 細胞障害 |
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| Outline of Final Research Achievements |
We investigated the cytotoxic effect of Cholix on HepG2 cells in the presence or absence of an inflammatory cytokine. Cytotoxicity of Cholix was dramatically enhanced with a cytokine in HepG2 cells. The mechanism of Cholix-induced cell death was involved in the signal transduction of MAPK, PKC or AMPK. In addition, we made a new peptide antibody for Cholix to identify the receptors on cell surface by immunoprecipitation. After biotin-labeled cell lysates were incubated with Cholix in the presence of anti-cholix peptide antibody, Cholix interaction proteins were detected by Western blotting using avidin-HRP. We observed two bands, which proteins were analyzed by TOF-Ms/Ms analysis.However, we could not identify these proteins. Now, we collect these bands to analyze again.
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Report
(4 results)
Research Products
(3 results)
