• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The comprehensive study of bacterial IpaH family effectors

Research Project

Project/Area Number 25460527
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including mycology)
Research InstitutionThe University of Tokyo

Principal Investigator

Ashida Hiroshi  東京大学, 医科学研究所, 特任准教授 (10535115)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords赤痢菌 / エフェクター / 自然免疫 / I型インターフェロン
Outline of Final Research Achievements

In this study, we tried to identify the mechanism of IpaH family effector proteins, which have E3 ubiquitin ligase activity, in Shigella infection. One of IpaH family protein, IpaH0722 inhibits NF-κB activity in its E3 ubiquitin ligase dependent manner. As a result, we found that IpaH0722 inhibits phagosome disruption mediated PKC-NF-κB activation by targeting TRAF2 for ubiquitination and proteasome-dependent degradation, thereby downregulating host inflammatory responses.In addition, another IpaH family protein, IpaH5 inhibits DNA dependent type I interferon signaling by targeting signaling factor X for ubiquitination.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (15 results)

All 2016 2015 2014 2013 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] Shigella IpaH family effectors as a versatile model for studying pathogenic bacteria2016

    • Author(s)
      Ashida H & Sasakawa C
    • Journal Title

      Front Cell. Infect. Microbiol

      Volume: 5 Pages: 100-100

    • DOI

      10.3389/fcimb.2015.00100

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Shigella manipulates host immune responses by delivering effector proteins with specific roles2015

    • Author(s)
      Ashida H, Mimuro H, & Sasakawa C
    • Journal Title

      Front Immunol

      Volume: 6 Pages: 219-219

    • DOI

      10.3389/fimmu.2015.00219

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Shigella manipulates host immune responses by delivering effector proteins with specific roles2015

    • Author(s)
      Ashida H, Mimuro H, Sasakawa C
    • Journal Title

      Frontier Immunology

      Volume: In press

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Shigella hacks host immune responses by reprogramming the host epigenome2014

    • Author(s)
      Ashida H, Sasakawa C
    • Journal Title

      EMBO J

      Volume: 33 Issue: 22 Pages: 2598-600

    • DOI

      10.15252/embj.201489934

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Manipulation of the host cell death pathway by Shigella2014

    • Author(s)
      Ashida H, Kim M, Sasakawa C
    • Journal Title

      Cell Microbiol

      Volume: 16 Issue: 12 Pages: 1757-66

    • DOI

      10.1111/cmi.12367

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Shigella IpaH7.8 E3 ligase targets glomulin and activates inflammasomes to demolish macrophages2014

    • Author(s)
      Suzuki S et.al
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 111 Issue: 40

    • DOI

      10.1073/pnas.1324021111

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Exploitation of the host ubiquitin system by human bacterial pathogens2014

    • Author(s)
      Ashida H, Kim M, & Sasakawa C
    • Journal Title

      Nature Reviews Microbiology

      Volume: 12 Pages: 1-15

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Shigella IpaH0722 E3 ubiquitin ligase effector targets TRAF2 to inhibit PKC-NF-κB activity in invaded epithelial cells.2013

    • Author(s)
      Ashida H, Nakano H, Sasakawa C.
    • Journal Title

      PLoS Pathog.

      Volume: 9 Issue: 6 Pages: e1003409-e1003409

    • DOI

      10.1371/journal.ppat.1003409

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Shigella OspC3 effector inhibits Cspase-4, antagonizes inflammatory cell death, and promotes epithelial infection2013

    • Author(s)
      Kobayashi T, et. al
    • Journal Title

      Cell Host Microbe

      Volume: 13 Issue: 5 Pages: 570-583

    • DOI

      10.1016/j.chom.2013.04.012

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Shigella manipulates host immune responses by delivering IpaH effector2015

    • Author(s)
      Hiroshi Ashida & Chihiro Sasakawa
    • Organizer
      COLD SPRING HARBOR ASIA CONFERENCES
    • Place of Presentation
      Suzhou, China
    • Year and Date
      2015-11-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Shigella manipulates host immune responses by delivering effector proteins with specific roles2015

    • Author(s)
      Hiroshi Ashida & Chihiro Sasakawa
    • Organizer
      The 14th Awaji International Forum on Infection and Immunity
    • Place of Presentation
      淡路島夢舞台国際展示場(兵庫県淡路市)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Shigella effector protein hacks host immune responses by targeting type I interferon signaling.2015

    • Author(s)
      Hiroshi Ashida & Chihiro Sasakawa
    • Organizer
      第88回 日本細菌学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] Shigella effector protein exploits host ubiquitin system2014

    • Author(s)
      Hiroshi Ashida & Chihiro Sasakawa
    • Organizer
      第3回感染症若手フォーラム
    • Place of Presentation
      長崎
    • Related Report
      2013 Research-status Report
  • [Presentation] 赤痢菌エフェクターによる宿主細胞の機能攪乱

    • Author(s)
      芦田 浩、笹川 千尋
    • Organizer
      第87回 細菌学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] 感染・炎症・免疫2013

    • Author(s)
      芦田 浩、笹川 千尋
    • Publisher
      医薬の門社
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi