Identification of bacterial virulent factor for whooping-cough development

• Project/Area Number: 25460529
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Osaka Prefecture University
• Principal Investigator: Kamitani Shigeki 大阪府立大学, 総合リハビリテーション学部, 教授 (60379089)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 病原性 / 百日咳

Outline of Final Research Achievements

It is found that a gene possibly encoding a transcription factor, which controls transcription of genome DNA to mRNA, is responsible for bacterial virulent factor of coughing by intranasal infection of rats with Bordetella bronchiseptica as an experimental model of whooping cough.

Research Products

• [Journal Article] Detection of genes expressed in Bordetella bronchiseptica colonizing rat trachea by in vivo expressed-tag immunoprecipitation method. (2015)
  • Author(s): Abe H, Kamitani S, Fukui-Miyazaki A, Shinzawa N, Nakamura K, and Horiguchi Y.
  • Journal Title: Microbiology and Immunology Volume: 印刷中 Issue: 5 Pages: 249-261
  • DOI: 10.1111/1348-0421.12247
  • NAID: 40020479086
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Induction of neutral lipid-containing granules by staphylococcal triacylglycerol lipase in cultured cells. (2014)
  • Author(s): Kamitani S, Miyake M, Hatano M, Yutsudo T, Minamide W, Kato I, and Noda M.
  • Journal Title: SOJ Microbiology and Infectious Diseases Volume: 2 Issue: 2 Pages: 1-7
  • DOI: 10.15226/sojmid.2014.00117
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Structural Biology of the Food Poisoning <i>Clostridium Perfringens</i> Enterotoxin (2013)
  • Author(s): 北所健悟, 西村昂亮, 神谷重樹, 堀口安彦
  • Journal Title: Nihon Kessho Gakkaishi Volume: 55 Issue: 3 Pages: 223-229
  • DOI: 10.5940/jcrsj.55.223
  • NAID: 10031183720
  • ISSN: 0369-4585, 1884-5576
  • Peer Reviewed
• [Presentation] 百日咳の病態発生機序解明に向けた感染動物モデルの確立 (2016)
  • Author(s): 中村佳司, 神谷重樹, 安倍裕順, 新澤直明, 堀口安彦
  • Organizer: 第８９回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター（大阪府大阪市）
  • Year and Date: 2016-03-23
• [Presentation] 歯周病原細菌の増殖に対して食品添加物が及ぼす効果 (2016)
  • Author(s): 篠原舞、前谷（安井）実希、矢澤彩香、神谷重樹
  • Organizer: 第８９回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター（大阪府大阪市）
  • Year and Date: 2016-03-23
• [Presentation] IVET-IP法: ラット感染中の気管支敗血症菌の遺伝子発現プロファイル解析 (2014)
  • Author(s): 安倍裕順，神谷重樹，福井理，岡田圭祐，堀口安彦
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京 タワーホール船堀
• [Presentation] ボルデテラ属細菌が産生するアデニレートサイクラーゼ毒素 (ACT)の菌種間における毒素作用の違いについて (2013)
  • Author(s): 福井理, 戸嶋ひろ野, 安倍裕順, 神谷重樹, 堀口安彦
  • Organizer: 第60回毒素シンポジウム
  • Place of Presentation: 兵庫県宍粟市 楓香荘