| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The bacterial pathogen Legionella pneumophila delivers a large array of the effector proteins into infected host cells using the type IVB secretion system. Among the proteins composing the system, inner membrane proteins DotI and DotJ form a hetero subcomplex that is anticipated to work as a transport channel of the IVB secretion system. We solved the crystal structure of the periplasmic domain of DotI and conducted functional analyses using the structural information. Bacterial localization analyses suggested that the DotI/DotJ complex can associate with the core complex of the type IVB secrestion system on demand. In addition, we identified a bacterial ATPase that associates with the DotI/DotJ complex and is essential for the secretion function. These findings support our hypothesis that the DotI/DotJ complex plays a role crucial for the IVB secretion utilizing the ATP energy.
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