Functional analysis of Vibrio parahaemolyticus T3SS2

• Project/Area Number: 25460531
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Osaka University
• Principal Investigator: KODAMA TOSHIO 大阪大学, 微生物病研究所, 准教授 (20346133)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 腸炎ビブリオ / エフェクター / 3型分泌装置 / 下痢原性

Outline of Final Research Achievements

In this study, I aimed to determine a pathogenic mechanism of food-borne pathogen, Vibrio parahaemolyticus.
I found that V. parahaemolyticus has two distinct mechanisms of diarrhea, T3SS2-effector (VopV) and T3SS2-toxin (TDH), assessed by rabbit ileal loop model. Hemolytic activity of TDH was necessary for T3SS2-TDH mediated enterotoxicity. All T3SS2 effectors that have already identified did not involve in this enterotoxicity. Therefore, we tried to identify new effector by proteomic approach and have gotten one candidate protein. The involvement of this effector candidate protein in T3SS2-TDH mediated enterotoxicity is currently under investigation.

Research Products

• [Journal Article] Genetic diversity of Vibrio parahaemolyticus strains isolated from farmed Pacific white shrimp and ambient pond water affected by acute hepatopancreatic necrosis disease outbreak in Thailand (2016)
  • Author(s): Chonsin K, Matsuda S, Theethakaew C, Kodama T, Junjhon J, Suzuki Y, Suthienkul O, Iida T
  • Journal Title: FEMS Microbiol Lett Volume: 363 Issue: 2 Pages: fnv222-fnv222
  • DOI: 10.1093/femsle/fnv222
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A repeat unit of Vibrio diarrheal T3S effector subverts cytoskeletal actin homeostasis via binding to interstrand region of actin filaments. (2015)
  • Author(s): Nishimura M, Fujii T, Hiyoshi H, Makino F, Inoue H, Motooka D, Kodama T, Ohkubo T, Kobayashi Y, Nakamura S, Namba K, Iida T.
  • Journal Title: Sci Rep. Volume: 5 Issue: 1 Pages: 10870-10870
  • DOI: 10.1038/srep10870
  • Peer Reviewed / Open Access
• [Journal Article] Interaction between the Type III Effector VopO and GEF-H1 Activates the RhoA-ROCK Pathway. (2015)
  • Author(s): Hiyoshi H, Okada R, Matsuda S, Gotoh K, Akeda Y, Iida T, Kodama T*
  • Journal Title: PLoS Pathogens Volume: 11(3) Issue: 3 Pages: e1004694-e1004694
  • DOI: 10.1371/journal.ppat.1004694
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Regulation of Vibrio parahaemolyticus T3SS2 gene expression and function of T3SS2 effectors that modulate actin cytoskeleton. (2015)
  • Author(s): Kodama T, Hiyoshi H, Okada R, Matsuda S, Gotoh K, Iida T.
  • Journal Title: Cellular Microbiology Volume: 17(2) Issue: 2 Pages: 183-90
  • DOI: 10.1111/cmi.12408
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Vibrio parahaemolyticus effector VopC mediates Cdc42-dependent invasion of cultured cells but is not required for pathogenicity in an animal model of infection (2014)
  • Author(s): Okada R, Zhou X, Hiyoshi H, Matsuda S, Chen X, Akeda Y, Kashimoto T, Davis BM, Iida T, Waldor MK, Kodama T
  • Journal Title: Cell Microbiol Volume: 16 Issue: 6 Pages: 938-947
  • DOI: 10.1111/cmi.12252
  • Peer Reviewed / Open Access
• [Journal Article] BEC, a novel enterotoxin of Clostridium perfringens found in human clinical isolates from acute gastroenteritis outbreaks (2014)
  • Author(s): Shinya Yonogi, Shigeaki Matsuda, Takao Kawai, Tomoko Yoda, Tetsuya Harad, Yuko Kumeda, Kazuyoshi Gotoh, Hirotaka Hiyoshi, Shota Nakamura, Toshio Kodama, Tetsuya Iida
  • Journal Title: Infection and Immunity Volume: 82 Issue: 6 Pages: 999-999
  • DOI: 10.1128/iai.01759-14
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of all RND-type multidrug efflux transporters in Vibrio parahaemolyticus. (2013)
  • Author(s): Matsuo T, Nakamura K, Kodama T, Mikami T, Hiyoshi H, Tsuchiya T, Ogawa W, Kuroda T.
  • Journal Title: Microbiologyopen. Volume: 2(5) Issue: 5 Pages: 752-742
  • DOI: 10.1002/mbo3.100
  • Peer Reviewed
• [Journal Article] Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in Vibrio cholerae (2013)
  • Author(s): Morita M, Yamamoto S, Hiyoshi H, Kodama T,Okura M, Arakawa E, Alam M, Ohnish M,Izumiya H, Watanabe H
  • Journal Title: Microbiol Immunol Volume: 57(5) Issue: 5 Pages: 334-9
  • DOI: 10.1111/1348-0421.12039
  • Peer Reviewed
• [Presentation] Actin cytoskeleton manipulating effectors of Vibrio parahaemolyticus (2016)
  • Author(s): 児玉年央、日吉大貴、岡田龍、松田重輝、後藤和義、飯田哲也
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター(大阪)
  • Year and Date: 2016-03-23
  • Invited
• [Presentation] Interaction of Vibrio parahaemolyticus diarrheal T3SS effector VopV with actin filaments (2016)
  • Author(s): 日吉大貴、児玉年央、飯田哲也
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター(大阪)
  • Year and Date: 2016-03-23
• [Presentation] Interaction between the type III effector VopO and GEF-H1 activates the RhoA-ROCK pathway (2016)
  • Author(s): Toshio Kodama
  • Organizer: US-Japan Cooperative Medical Science Program
  • Place of Presentation: North Bethesda, Maryland, USA
  • Year and Date: 2016-01-11
  • Int'l Joint Research
• [Presentation] Identification and characterization of stress fiber inducing effector protein of Vibrio parahaemolyticus T3SS2 (2015)
  • Author(s): Toshio Kodama
  • Organizer: FEMS2015
  • Place of Presentation: Maastricht, The Netherlands
  • Year and Date: 2015-06-07
  • Int'l Joint Research
• [Presentation] 腸炎ビブリオT3SS2エフェクターVopOはRhoA-ROCK経路を活性化する (2015)
  • Author(s): 日吉大貴、岡田龍、松田重輝、後藤和義、明田幸宏、飯田哲也、児玉年央
  • Organizer: 第88回 日本細菌学会総会
  • Place of Presentation: 長良川国際会議場（岐阜県岐阜市）
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] ウェルシュ菌新規エンテロトキシン BEC(Binary Enterotoxin of Clostridium perfringens)の同定 (2014)
  • Author(s): 余野木 伸哉、松田 重輝、河合 高生、依田 知子、原田 哲也、久米田 裕子、後藤 和義、日吉 大貴、中村 昇太、児玉 年央、 飯田 哲也
  • Organizer: 第67回 日本細菌学会関西支部総会
  • Place of Presentation: 兵庫医科大学（兵庫県西宮市）
  • Year and Date: 2014-11-22
• [Presentation] T3SS2を介したアクチンストレスファイバー形成機構の解析 (2014)
  • Author(s): 日吉大貴、岡田龍、松田重輝、後藤和義、明田幸宏、飯田哲也、児玉年央
  • Organizer: 第48回 腸炎ビブリオシンポジウム
  • Place of Presentation: 金森ホール（北海道函館市）
  • Year and Date: 2014-11-13 – 2014-11-14
• [Presentation] The Vibrio parahaemolyticus effector VopC mediates Cdc42 dependent invasion of cultured cells but is not required for pathogenicity in an animal model of infection (2014)
  • Author(s): Toshio Kodama
  • Organizer: American Society for Microbiology 114th General Meeting
  • Place of Presentation: Boston Convention and Exhibition Center (Boston, USA)
  • Year and Date: 2014-05-17 – 2014-05-20
• [Presentation] VopV, an F-actin-binding Type III secretion effector, is required for Vibrio parahaemolyticus-induced enterotoxicity (2014)
  • Author(s): Hiyoshi H, Kodama T, Iida T.
  • Organizer: Vibrio 2014
  • Place of Presentation: Edinburgh, UK
  • Invited
• [Presentation] Functional analysis of Vibrio parahaemolyticus T3SS2 effectors. (2014)
  • Author(s): Kodama T.
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 東京
  • Invited
• [Presentation] 腸炎ビブリオT3SS2エフェクターVopCの機能解析 (2014)
  • Author(s): 岡田龍、日吉大貴、松田重輝、明田幸広、柏本孝茂、児玉年央、飯田哲也
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 東京
• [Presentation] 腸炎ビブリオT3SS2エフェクターVopCの細胞侵入およびストレスファイバー形成への関与 (2013)
  • Author(s): 岡田龍、日吉大貴、松田重輝、明田幸広、柏本孝茂、児玉年央、飯田哲也
  • Organizer: 日本細菌学会関西支部総会
  • Place of Presentation: 大阪
• [Presentation] 腸炎ビブリオT3SS2エフェクターVopCの細胞侵入およびストレスファイバー形成への関与 (2013)
  • Author(s): 岡田龍、日吉大貴、松田重輝、明田幸広、柏本孝茂、児玉年央、飯田哲也
  • Organizer: 腸炎ビブリオシンポジウム
  • Place of Presentation: 広島
• [Presentation] An novel F-actin-binding Type III secretion effector is required for Vibrio parahaemolyticus-induced enterotoxicity. (2013)
  • Author(s): Kodama T, Hiyoshi H, Saito K, Gotoh K, Matsuda S, Akeda Y, Honda T, Iida T.
  • Organizer: FEMS 2013
  • Place of Presentation: Leipzig, Germany
• [Presentation] Relationship between localization on cellular membranes and cytotoxicity of Vibrio vulnificus hemolysin. (2013)
  • Author(s): Kashimoto T, Sugiyama H, Ueno S, Ehara H, Kodama T, Iida T, Susa N.
  • Organizer: FEMS 2013
  • Place of Presentation: Leipzig, Germany
• [Book] 腸炎ビブリオ 第IV集 (2013)
  • Author(s): 本田武司／監修 児玉年央／分担
  • Total Pages: 362
  • Publisher: 近代出版
• [Remarks] 腸炎ビブリオのT3SS2エフェクターVopOはRhoA-ROCK経路を活性化する
  • URL: http://www.biken.osaka-u.ac.jp/topics/more.html?paper=4142