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Roles of retron and msDNA in regulation of virulence expression and genome evolution

Research Project

Project/Area Number 25460532
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including mycology)
Research InstitutionHiroshima University

Principal Investigator

Shimamoto Tadashi  広島大学, 生物圏科学研究科, 教授 (90187443)

Co-Investigator(Kenkyū-buntansha) SHIMAMOTO TOSHI  広島大学, 大学院生物圏科学研究科, 助教 (70583136)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords病原性 / レトロン / コレラ菌 / msDNA / ビブリオ / ビブリオ属
Outline of Final Research Achievements

Relationship between pathogenicity and retron-Vc95 in Vibrio choelare O1/O139 serotypes has been investigated in this study. Candidate proteins that can interact with ORF540 or ORF205, encoded by the genes located downstream of the ret gene, of unknown functions were searched. One of the candidates, ArcB that regulates genes related to anaerobic respiration, was found. The arcB-defective mutant was found to have lower pathogenicity to silk warm larvae than the wild type. VC0176 proten, one of the candidate proteins that can bind to msDNA-Vc95, was purified. Binding of the VC0176 protein to the promoter region of VC0177 gene and msDNA-Vc95 was confirmed.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (25 results)

All 2016 2015 2014 2013 Other

All Journal Article (12 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 12 results,  Acknowledgement Compliant: 8 results) Presentation (11 results) Book (2 results)

  • [Journal Article] Emergence of plasmid-mediated colistin resistance gene, mcr-1, in a clinical Escherichia coli isolate from Egypt2016

    • Author(s)
      Shimaa S. Elnahriry, Hazim O. Khalifa, Ahmed M. Soliman, Ashraf M. Ahmed, Alaaddin M. Hussein, Toshi Shimamoto, Tadashi Shimamoto
    • Journal Title

      Antimicrobial Agents and Chemotherapy

      Volume: 60 Issue: 5 Pages: 3249-3250

    • DOI

      10.1128/aac.00269-16

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Characterization of plasmid-mediated colistin resistance gene, mcr-1, in Escherichia coli isolated from animals in Egypt2016

    • Author(s)
      Hazim O. Khalifa, Ashraf M. Ahmed, Atef F. Oreiby, Amal M. Eid, Toshi Shimamoto, Tadashi Shimamoto
    • Journal Title

      International Journal of Antimicrobial Agents

      Volume: in press Issue: 5 Pages: 413-414

    • DOI

      10.1016/j.ijantimicag.2016.02.011

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] NDM-4- and NDM-5-producing Klebsiella pneumoniae coinfection in a 6-month-old infant2016

    • Author(s)
      Hazim O. Khalifa, Ahmed M. Soliman, Ashraf M. Ahmed, Toshi Shimamoto, Tadashi Shimamoto
    • Journal Title

      Antimicrobial Agents and Chemotherapy

      Volume: 60 Issue: 7 Pages: 4416-4417

    • DOI

      10.1128/aac.00479-16

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Emergence of NDM-5-producing clinical Escherichia coli isolate in Egypt2016

    • Author(s)
      Ahmed M. Soliman, Hazim O. Khalifa, Ashraf M. Ahmed, Toshi Shimamoto, Tadashi Shimamoto
    • Journal Title

      International Journal of Infectious Diseases

      Volume: in press Pages: 46-48

    • DOI

      10.1016/j.ijid.2016.05.003

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Seafood as a reservoir of Gram-negative bacteria carrying integrons and antimicrobial resistance genes in Japan2015

    • Author(s)
      Ashraf M. Ahmed, Akito Maruyama, Hazim O. Khalifa, Toshi Shimamoto, Tadashi Shimamoto
    • Journal Title

      Biomedical and Environmental Sciences

      Volume: 28 Pages: 924-927

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Molecular analysis of multidrug resistance in Shiga toxin-producing Escherichia coli O157:H7 isolated from meat and dairy products2015

    • Author(s)
      Ashraf M. Ahmed, Tadashi Shimamoto
    • Journal Title

      International Journal of Food Microbiology

      Volume: 193 Pages: 68-73

    • DOI

      10.1016/j.ijfoodmicro.2014.10.014

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Prevalence, antibiotic resistance and virulence of Enterococcus spp. in Egyptian raw milk cheese2015

    • Author(s)
      Ahmed M. Hammad, Hamdy A. Hassan, Tadashi Shimamoto
    • Journal Title

      Food Control

      Volume: 50 Pages: 815-820

    • DOI

      10.1016/j.foodcont.2014.10.020

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Molecular characterization of multidrug-resistant Shigella spp. of food origin2015

    • Author(s)
      Ashraf M. Ahmed, Tadashi Shimamoto
    • Journal Title

      International Journal of Food Microbiology

      Volume: 194 Pages: 78-82

    • DOI

      10.1016/j.ijfoodmicro.2014.11.013

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Characterization of integrons and resistance genes in multidrug-resistant Salmonella enterica isolated from meat and dairy products in Egypt2014

    • Author(s)
      Ashraf M. Ahmed, Toshi Shimamoto, Tadashi Shimamoto
    • Journal Title

      International Journal of Food Microbiology

      Volume: 189 Pages: 39-44

    • DOI

      10.1016/j.ijfoodmicro.2014.07.031

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli isolated from septicemic broilers2014

    • Author(s)
      Ashraf M. Ahmed, Toshi Shimamoto, Tadashi Shimamoto
    • Journal Title

      International Journal of Medical Microbiology

      Volume: 303 Issue: 8 Pages: 475-483

    • DOI

      10.1016/j.ijmm.2013.06.009

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genetic characterization of antibiotic resistance and virulence factors in Enterococcus spp. from Japanese retail ready-to-eat raw fish2014

    • Author(s)
      Ahmed M. Hammad, Toshi Shimamoto, Tadashi Shimamoto
    • Journal Title

      Food Microbiology

      Volume: 38 Pages: 62-66

    • DOI

      10.1016/j.fm.2013.08.010

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Isolation and molecular characterization of Salmonella enterica, Escherichia coli O157:H7 and Shigella spp. from meat and dairy products in Egypt2014

    • Author(s)
      Ashraf M. Ahmed, Tadashi Shimamoto
    • Journal Title

      International Journal of Food Microbiology

      Volume: 168-169 Pages: 57-62

    • DOI

      10.1016/j.ijfoodmicro.2013.10.014

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] コレラ菌の病原性発現調節におけるレトロンとmsDNAの機能解析2016

    • Author(s)
      湯ノ谷 学,島本 敏,島本 整
    • Organizer
      第89回日本細菌学会総会
    • Place of Presentation
      大阪市
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] Characterization of msDNA binding proteins in Vibrio cholerae2015

    • Author(s)
      Toshi Shimamoto, Gaku Yunotani, Tadashi Shimamoto
    • Organizer
      日米医学協力研究会コレラ・細菌性腸管感染症専門部会 2015年度日本側総会
    • Place of Presentation
      京都市
    • Year and Date
      2015-07-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] O1エルトール型コレラ菌のレトロン-Vc95内機能未知遺伝子orf540の機能解析2015

    • Author(s)
      エン 智群,島本 敏,湯ノ谷 学,成谷宏文,桑原知巳,島本 整
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      長良川国際会議場(岐阜市)
    • Year and Date
      2015-03-26 – 2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] コレラ菌のレトロン-Vc95内機能未知遺伝子orf540の古典型とエルトール型における機能の違い2015

    • Author(s)
      井本圭祐,島本 敏,エン 智群,湯ノ谷 学,島本 整
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      長良川国際会議場(岐阜市)
    • Year and Date
      2015-03-26 – 2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] レトロンとmsDNAによるコレラ菌(Vibrio cholerae)の病原性発現制御機構2014

    • Author(s)
      湯ノ谷 学,島本 敏,神本真紀,島本 整
    • Organizer
      第67回日本細菌学会中国・四国支部総会
    • Place of Presentation
      徳島文理大学(徳島市)
    • Year and Date
      2014-10-04
    • Related Report
      2014 Research-status Report
  • [Presentation] Roles of retron-Vc95 in virulence regulation of Vibrio cholerae2014

    • Author(s)
      Gaku Yunotani, Toshi Shimamoto, Zhiqun Yan, Maki Kamimoto, Tadashi Shimamoto
    • Organizer
      日米医学協力研究会コレラ・細菌性腸管感染症専門部会 2014年度日本側総会
    • Place of Presentation
      京都大学稲盛財団記念館(京都市)
    • Year and Date
      2014-08-07
    • Related Report
      2014 Research-status Report
  • [Presentation] O1エルトール型コレラ菌のレトロン-Vc95内機能未知遺伝子orf205の機能解析2014

    • Author(s)
      島本 敏,成谷 宏文,桑原 知巳,島本 整
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Related Report
      2013 Research-status Report
  • [Presentation] VBNC状態のVibrio parahaemolyticusに関する抗酸化酵素を用いた復帰条件の検討2014

    • Author(s)
      大楽 菜穂,神本 真紀,島本 敏,島本 整
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀(東京都江戸川区)
    • Related Report
      2013 Research-status Report
  • [Presentation] Plasmid-derived retron of Escherichia coli clinical isolate is closely related to retron of Salmonella enterica serovar Enteritidis

    • Author(s)
      Miho Kawabata, Maki Kamimoto, Toshi Shimamoto, Tadashi Shimamoto
    • Organizer
      日米医学協力研究会コレラ・細菌性腸管感染症専門部会 日本側総会
    • Place of Presentation
      京都大学稲盛財団記念館(京都市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 市販の刺身より分離した腸球菌の薬剤感受性と病原性遺伝子の解析

    • Author(s)
      島本 整,島本 敏
    • Organizer
      第66回 日本細菌学会中国・四国支部総会
    • Place of Presentation
      広島国際大学呉キャンパス(広島県呉市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 変異株を利用したVibrio mimicusのレトロンの機能解析

    • Author(s)
      田鳥 安紗子,島本 敏,島本 整
    • Organizer
      第47回腸炎ビブリオシンポジウム
    • Place of Presentation
      広島大学学士会館(広島県東広島市)
    • Related Report
      2013 Research-status Report
  • [Book] 腸炎ビブリオ〈第IV集〉2013

    • Author(s)
      島本整,島本敏
    • Total Pages
      362
    • Publisher
      近代出版
    • Related Report
      2013 Research-status Report
  • [Book] Bacterial Satellite DNA: A Novel Dimension in Bacterial Pathogenicity2013

    • Author(s)
      Rasel Das, Tadashi Shimamoto, Mohammad Arifuzzaman
    • Total Pages
      74
    • Publisher
      LAMBERT Academic Publishing
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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