| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Protein Ser/Thr Kinase (K)-Phosphatase (P) plays essential roles in regulation of cellular functions in both prokaryotes and eukaryotes. Although PP2C family P- PknB family receptor K system is conserved in Firmicutes bacteria, functional diversity of the system is implicated by its substrate (S) diversity.
Our studies on the K-P system in C. perfringens revealed that S is a novel signaling factor distributing only in Clostridia and containg protein-protein interaction domains. S is phosphorylated at Thr-92 and the phosphorylated-form S is increased by according to cell-growth progression. Cell-length is corelated with the phosphorylation level of S upon the K-P system. Furthermore, S is located at septa and poles in the cells, probably associating with two essential enzymes in the cell wall synthesis pathways.
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