Study on Function of Eukaryotic Protein Kinase and Phosphatase in Firmicutes
| Project/Area Number |
25460535
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Kagawa University |
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Principal Investigator |
NARIYA HIROFUMI 香川大学, 医学部, 助教(学内講師) (30452668)
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| Co-Investigator(Kenkyū-buntansha) |
TAMAI EIJI 松山大学, 薬学部, 准教授 (40333512)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ガス壊疽菌 / シグナル伝達 / リン酸化 / プロテインキナーゼ / プロテインホスファターゼ / Clostridium perfringens / 形態形成 / 多様性 / 非メバロン酸経路 / 細胞壁生合成経路 / Yeast Two-Hybrid Screen / タンパク質相互作用 |
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| Outline of Final Research Achievements |
Protein Ser/Thr Kinase (K)-Phosphatase (P) plays essential roles in regulation of cellular functions in both prokaryotes and eukaryotes. Although PP2C family P- PknB family receptor K system is conserved in Firmicutes bacteria, functional diversity of the system is implicated by its substrate (S) diversity.
Our studies on the K-P system in C. perfringens revealed that S is a novel signaling factor distributing only in Clostridia and containg protein-protein interaction domains. S is phosphorylated at Thr-92 and the phosphorylated-form S is increased by according to cell-growth progression. Cell-length is corelated with the phosphorylation level of S upon the K-P system. Furthermore, S is located at septa and poles in the cells, probably associating with two essential enzymes in the cell wall synthesis pathways.
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Report
(4 results)
Research Products
(37 results)
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[Journal Article] DNA inversion regulates outer membrane vesicle production in Bacteroides fragilis.2016
Author(s)
Nakayama-Imaohji H, Hirota K, Yamasaki H, Yoneda S, Nariya H, Suzuki M, Secher T, Miyake Y, Oswald E, Hayashi T, Kuwahara T
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Journal Title
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] X-ray structure of a novel endolysin encoded by episomal phage phiSM101 of Clostridium perfringens.2014
Author(s)
Tamai, E., Yoshida, H., Sekiya, H., Nariya, H., Miyata, S., Okabe, A., Kuwahara, T., Maki, J. & Kamitori, S
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Journal Title
Mol Microbiol.
Volume: 92
Pages: 326-337
DOI
Related Report
Peer Reviewed
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[Presentation] Identification of two cell wall–associated fibronectin-binding proteins of Clostridium perfringens2015
Author(s)
Ayumu Yamasaki, Sanae Kato, Kana Aitani, Tsutomu. Yamasaki, Naoya Hatano, Hirofumi Nariya, Yasuo Hitsumoto, Seiichi Katayama
Organizer
CLOSTPATH 2015, 9th International Conference on the Molecular Biology and Pathogenesis of the Clostridia.
Place of Presentation
Hotel Schloss Reinach, Freiburg, Germany
Year and Date
2015-09-07
Related Report
Int'l Joint Research
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[Presentation] Determination of binding site for Clostridium perfringens on fibronectin2013
Author(s)
Seiichi Katayama, Mika Tagomori, Hatsuo Yamashita, Tsutomu Yamasaki, Hirofumi Nariya, Mariko Okada, Mariko Watanabe, and Yasuo Hitsumoto
Organizer
8th International Conference on the Molecular Biology and Pathogenesis of the Clostridia 2013
Place of Presentation
Sea Temple Resort & Spa Palm Cove, Palm Cove, Queensland, Australia
Related Report
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