Identification of high risk lineages that contain Stx2 high producing strains in enterohaemorrhagic Escherichia coli
Project/Area Number |
25460542
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Bacteriology (including mycology)
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Research Institution | Kyushu University (2015) University of Miyazaki (2013-2014) |
Principal Investigator |
Yoshitoshi Ogura 九州大学, 医学(系)研究科(研究院), 准教授 (40363585)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 腸管出血性大腸菌 / 志賀毒素 / O157 / ファージ / ゲノム / non-O157 EHEC |
Outline of Final Research Achievements |
Enterohemorrhagic E. coli (EHEC) causes diarrhea and hemorrhagic colitis with life-threatening complications, such as hemolytic uremic syndrome. Their major virulence factor is Shiga toxin (Stx), which is encoded by bacteriophages. Of the two types of Stx, the production of Stx2, particularly that of Stx2a (a subtype of Stx2), is a major risk factor for severe EHEC infections, but the Stx2 production level is highly variable between strains. Here, we define four major subtypes of Stx2a-encoding phages according to their genomic structure. The subtypes are correlated with Stx2a titers produced by the host O157 strains, suggesting a critical role of the phage subtype in determining the Stx2a production level. To identify high risk EHEC lineage, we performed genome sequence, high-resolution phylogenetic analysis and determination of Stx2 production of 300, 50, and 50 strains of O157, O145 and O121, respectively. By this analysis, we identified 3 high risk O157 lineages.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Genetic relatedness and virulence properties of enteropathogenic Escherichia coli strains of serotype O119:H6 expressing localized adherence or localized and aggregative adherence-like patterns on HeLa cells.2016
Author(s)
Garcia BG, Ooka T, Gotoh Y, Vieira MA, Yamamoto D, Ogura Y, Girão DM, Sampaio SC, Melo AB, Irino K, Hayashi T, Gomes TA.
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Journal Title
Int J Med Microbiol.
Volume: 27
Issue: 3
Pages: 152-164
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Identification of Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli in diarrhoeic calves and comparative genomics of O5 bovine and human STEC.2016
Author(s)
Fakih I, Thiry D, Duprez JN, Saulmont M, Iguchi A, Pirard D, Jouant L, Daube G, Ogura Y, Hayashi T, Taminiau B, Mainil JG.
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Journal Title
Vet Microbiol.
Volume: 23
Pages: 16-22
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] The Shiga toxin 2 production level in enterohemorrhagic Escherichia coli O157:H7 is correlated with the subtypes of toxin-encoding phage.2015
Author(s)
Yoshitoshi Ogura, Shakhinur Islam Mondal, Md Rakibul Islam, Toshihiro Mako, Kokichi Arisawa, Keisuke Katsura, Tadasuke Ooka, Yasuhiro Gotoh, Kazunori Murase, Makoto Ohnishi, and Tetsuya Hayashi
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Journal Title
Scientific Reports
Volume: 5
Issue: 1
Pages: 16663-16663
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Efficient de novo assembly of highly heterozygous genomes from whole-genome shotgun short reads.2014
Author(s)
R. Kajitani, K. Toshimoto, H. Noguchi, A. Toyoda, Y. Ogura, M. Okuno, M. Yabana, M. Harada, E. Nagayasu, H. Maruyama, Y. Kohara, A. Fujiyama, T. Hayashi and T.Itoh
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Journal Title
Genome Research,
Volume: 24
Issue: 8
Pages: 1384-1384
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A novel small regulatory RNA enhances cell motility in enterohemorrhagic Escherichia coli.2014
Author(s)
Sudo N, Soma A, Muto A, Iyoda S, Suh M, Kurihara N, Abe H, Tobe T, Ogura Y, Hayashi T, Kurokawa K, Ohnishi M, Sekine Y.
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Journal Title
J Gen Appl Microbiol.
Volume: 60
Pages: 44-50
NAID
Related Report
Peer Reviewed
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[Journal Article] Phylogenetic Clades 6 and 8 of Enterohemorrhagic Escherichia coli O157:H7 With Particular stx Subtypes are More Frequently Found in Isolates From Hemolytic Uremic Syndrome Patients Than From Asymptomatic Carriers.2014
Author(s)
Iyoda S, Manning SD, Seto K, Kimata K, Isobe J, Etoh Y, Ichihara S, Migita Y, Ogata K, Honda M, Kubota T, Kawano K, Matsumoto K, Kudaka J, Asai N, Yabata J, Tominaga K, Terajima J, Morita-Ishihara T, Izumiya H, Ogura Y, et al.
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Journal Title
Open Forum Infect Dis.
Volume: 12
Issue: 2
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Identification of high Stx2-producing Escherichia coli O157 clades by WGS-based phylogenetic analysis2016
Author(s)
Yoshitoshi Ogura, Jun Yatsuyanagi, Kazuko Seto, Hiroshi Narimatsu, Kikuyo Ogata, Keiko Kimata, Junko Isobe, Eriko Maeda, Yoshiki Etoh, Mitsuhiro Kameyama, Shakhinur Islam Mondal, Md Rakibul Islam, Tadasuke Ooka, Yasuhiro Gotoh, Kazunori Murase, Makoto Ohnishi, and Tetsuya Hayashi
Organizer
SMBE Satellite Workshop on Genome Evolution in Pathogen Transmission and Disease
Place of Presentation
Shiga Lake Hotel, Nagano, Japan
Year and Date
2016-02-22
Related Report
Int'l Joint Research
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