| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Virulent and avirulent strains of group A streptococcus (GAS) emm type 1 were simultaneously isolated from a single patient suffering from streptococcal meningitis. The whole-genome sequencing and comparative genomic analysis revealed the possibility that a spontaneous point mutation in the rocA gene led to the hypervirulent phenotype of emm1 GAS associated with the suppression of covR expression. Meanwhile, the emm1 GAS strain GAS472 was isolated from a patient suffering from streptococcal toxic shock-like syndrome. CD46 transgenic (Tg) mice developed necrotizing fasciitis associated with osteoclast-mediated progressive and severe bone destruction in the hind paws after subcutaneous infection with GAS472. GAS472 infection induced expression of the receptor activator of nuclear factor-κB ligand (RANKL) in heterogeneous bone marrow cells. Therefore, GAS472 infection induced local bone erosion and systemic bone loss in CD46 Tg mice.
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