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Crosstalk between pifitlin-a and LPS-mediated inflammatory response

Research Project

Project/Area Number 25460551
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including mycology)
Research InstitutionAichi Medical University

Principal Investigator

Yokochi Takashi  愛知医科大学, 医学部, 名誉教授 (20126915)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsエンドトキシン / pyhin1 / MyD88非依存性 / NF-kappaB / インターフェロン-β / LPS / 一酸化窒素 / インターフェロンβ / PYHIN1 / マクロファージ
Outline of Final Research Achievements

The regulatory action of mouse pyhin1 on LPS-induced inflammatory response was examined. LPS augmented the pyhin1 mRNA expression in murine RAW 264.7 macrophage cells and peritoneal macrophages. The augmentation of pyhin1 mRNA expression was abolished by parthenolide, a NF-κB inhibitor. Silencing of pyhin1 with small interfering RNA reduced the production of IFN-β and NO. However, pyhin1 silencing did not affect the production of TNF-α, IL-6, IL-10 and prostaglandin E2. Reduced IFN-β production by pyhin1 silencing caused inactivation of STAT1 and reduced expression of IRF1. Pyhin1 silencing inhibited the expression of TRAF6, TBK1 and TRIF, which trigger IFN-β production in the MyD88-independent pathway. Taken together, mouse pyhin1 was suggested to be a NF-κB-responsible gene in response to LPS and positively regulate LPS-induced IFN-β and NO production through up-regulating the MyD88-independent signaling pathway.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (13 results)

All 2015 2014

All Journal Article (13 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 13 results,  Acknowledgement Compliant: 11 results)

  • [Journal Article] Lipopolysaccharide downregulates the expression of p53 through activation of MDM2 and enhances activation of nuclear factor-kappa B2015

    • Author(s)
      Odkhuu E, Mendjargal A, Koide N, Naiki Y, Komatsu T, Yokochi T
    • Journal Title

      Immunobiology

      Volume: 220 Issue: 1 Pages: 136-141

    • DOI

      10.1016/j.imbio.2014.08.010

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Involvement of redox balance in in vitro osteoclast formation of RAW 264.7 macrophage cells in response to LPS.2015

    • Author(s)
      Odkhuu E, Koide N, Tsolmongyn B, Jambalganiin U, Naiki Y, Komatsu T, Yoshida T, Yokochi T.
    • Journal Title

      Innate Immun.

      Volume: 21 Issue: 2 Pages: 194-202

    • DOI

      10.1177/1753425914524242

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Inhibition of RANKL- and LPS-induced osteoclast differentiations by novel NF-κB inhibitor DTCM-glutarimide.2015

    • Author(s)
      Koide N, Kaneda A, Yokochi T, Umezawa K.
    • Journal Title

      Int Immunopharmacol.

      Volume: 25 Issue: 1 Pages: 162-168

    • DOI

      10.1016/j.intimp.2015.01.004

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Poly I:C enhances production of nitric oxide in response to interferon-γ via upregulation of interferon regulatory factor 7 in vascular endothelial cells.2015

    • Author(s)
      Mori D, Koide N, Tsolmongyn B, Nagata H, Sano T, Nonami T, Yokochi T.
    • Journal Title

      Microvasc Res.

      Volume: 9 Pages: 68-73

    • DOI

      10.1016/j.mvr.2015.01.003

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Irbesartan attenuates production of high-mobility group box 1 in response to lipopolysaccharide via downregulation of interferon-β production.2015

    • Author(s)
      Kato Y, Kamiya H, Koide N, Odkhuu E, Komatsu T, Watarai A, Kondo M, Kato K, Nakamura J, Yokochi T.
    • Journal Title

      Int Immunopharmacol.

      Volume: 26 Issue: 1 Pages: 97-102

    • DOI

      10.1016/j.intimp.2015.03.015

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] TGF‐1 inhibits the production of IFN in response to CpG DNA via ubiquicination of TNF receptor-associated factor(TRAF)62015

    • Author(s)
      Naiki Y, Komatsu T, Koide N, Dagvadorji J, Yoshida T, Arditi M, Yokochi T.
    • Journal Title

      Innate Immun.

      Volume: 21 Issue: 7 Pages: 770-777

    • DOI

      10.1177/1753425915596844

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Pretreatment of LPS inhibits IFN-β-induced STAT1 phosphorylation through SACS3 induced by LPS2015

    • Author(s)
      Ando T, Komatsu T, Naiki Y, Yokochi T, Watanabe D, Koide N
    • Journal Title

      Biomed Pharmacother.

      Volume: 76 Pages: 1-5

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A novel mechanism for inhibition of lipopolysaccharide-induced proinflammatory cytokine production by valproic acid2014

    • Author(s)
      Jambalganiin U, Tsolmongyn B, Koide N, Odkhuu E, Naiki Y, Komatsu T, Yoshida T, Yokochi T
    • Journal Title

      Int Immunopharmacol

      Volume: 20 Issue: 1 Pages: 181-187

    • DOI

      10.1016/j.intimp.2014.02.032

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Inhibition of receptor activator of nuclear factor-κB ligand- or lipopolysaccharide-induced osteoclast formation by conophylline through downregulation of CREB.2014

    • Author(s)
      Koide N, Kondo Y, Odkhuu E, Ulziisaikhan J, Ukaji T, Yokochi T, Umezawa K.
    • Journal Title

      Immunol Lett.

      Volume: 161 Issue: 1 Pages: 31-37

    • DOI

      10.1016/j.imlet.2014.04.006

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Spironolactone inhibits production of proinflammatory mediators in response to lipopolysaccharide via inactivation of nuclear factor-κB2014

    • Author(s)
      Kato Y, Kamiya H, Koide N, Odkhuu E, Komatsu T, Dagvadorj J, Watarai A, Kondo, M, Kato K, Nakamura J, Yokochi T
    • Journal Title

      Immunopharmacol Immunotoxicol

      Volume: 36 Issue: 3 Pages: 237-241

    • DOI

      10.3109/08923973.2014.921690

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Sendai virus C protein inhibits lipopolysaccharide-induced nitric oxide production through impairing interferon-β signaling2014

    • Author(s)
      Odkhuu E, Komatsu T, Naiki Y, Koide N, Yokochi T
    • Journal Title

      Int Immunopharmacol

      Volume: 23 Issue: 1 Pages: 267-272

    • DOI

      10.1016/j.intimp.2014.09.012

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Mouse pyrin and HIN domain family member 1 (pyhin1) protein positively regulates LPS-induced IFN-β and NO production in macrophages2014

    • Author(s)
      Haque A, Koide N, Odkhuu E, Tsolmongyn B, Naiki Y, Komatsu T, Yoshida T, Yokochi T.
    • Journal Title

      Innate Immun

      Volume: 20 Issue: 1 Pages: 40-48

    • DOI

      10.1177/1753425913481636

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Lipopolysaccharide impairs insulin sensitivity via activation of phosphoinositide 3-kinase in adipocytes2014

    • Author(s)
      Wakayama S, Haque A, Koide N, Kato Y, Odkhuu E, Bilegtsaikhan T, Naiki Y, Komatsu T, Yoshida T, Yokochi T.
    • Journal Title

      Immunopharmacol Immunotoxicol.

      Volume: 36 Issue: 2 Pages: 145-149

    • DOI

      10.3109/08923973.2014.887096

    • Related Report
      2013 Research-status Report
    • Peer Reviewed

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Published: 2014-07-25   Modified: 2019-07-29  

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