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Amplification of a novel chromosomal resistance island of Salmonella Typhimurium confers increased resistance to antimicrobials

Research Project

Project/Area Number 25460553
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including mycology)
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

AKIBA Masato  国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門・細菌・寄生虫研究領域, ユニット長 (60355211)

Co-Investigator(Renkei-kenkyūsha) KURODA Makoto  国立感染症研究所, 病原体ゲノム解析研究センター, センター長 (80317411)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsSalmonella Typhimurium / 薬剤耐性 / ゲノミックアイランド / 多コピー化 / blaCMY-2
Outline of Final Research Achievements

We found that antimicrobial resistance of Salmonella Typhimurium increased by gene amplification of a novel chromosomal resistance island (GI). Copy number of GI increased up to 85 and the elevation was accompanied by increased amount of transcription. The amplification occurred at the original position. In some cases, the transposition of part of GI occurred after insertion of IS26 or IS1, leading to the amplification of GI at different position. Elevation of transcription of SOS response related genes was observed in a comprehensive gene expression analysis.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2015 2014 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (5 results)

  • [Journal Article] Extensive amplification of GI-VII-6, a multidrug resistance genomic island of Salmonella enterica serovar Typhimurium, increases resistance to extended-spectrum cephalosporins.2015

    • Author(s)
      Lee K, Kusumoto M, Sekizuka T, Kuroda M, Uchida I, Iwata T, Okamoto S, Yabe K, Inaoka T, Akiba M
    • Journal Title

      Front Microbiol

      Volume: 6 Pages: 78-78

    • DOI

      10.3389/fmicb.2015.00078

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Whole-Genome Sequence of CMY-2 beta-Lactamase-Producing Salmonella enterica Serovar Typhimurium Strain L-35532014

    • Author(s)
      Sekizuka T, Lee K, Kuroda M, Kusumoto M, Iwata T, Uchida I, Tanaka K, Tamamura Y, Akiba M
    • Journal Title

      Genome Announc

      Volume: 2 Issue: 4

    • DOI

      10.1128/genomea.00711-14

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] サルモネラ薬剤耐性アイランド多コピー化の様式解明2014

    • Author(s)
      李謙一、関塚剛史、黒田誠、楠本正博、内田郁夫、岩田剛敏、岡本晋、矢部希見子、稲岡隆史、秋庭正人
    • Organizer
      第157回日本獣医学会
    • Place of Presentation
      北海道大学(北海道・札幌市)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 多剤耐性Salmonella Typhimuriumのゲノム生物学的特徴2014

    • Author(s)
      関塚剛史、李謙一、黒田誠、楠本正博、岩田剛敏、内田郁夫、田中聖、玉村雪乃、秋庭正人
    • Organizer
      第157回日本獣医学会
    • Place of Presentation
      北海道大学(北海道・札幌市)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 薬剤耐性アイランドの多コピー化に基づくサルモネラの耐性増強

    • Author(s)
      李謙一、稲岡隆史、楠本正博、内田郁夫、岩田剛敏、岡本晋、矢部希見子、秋庭正人
    • Organizer
      第156回日本獣医学会学術集会
    • Place of Presentation
      岐阜大学
    • Related Report
      2013 Research-status Report
  • [Presentation] サルモネラ薬剤耐性アイランドの多コピー化に基づく耐性増強

    • Author(s)
      李謙一、稲岡隆史、楠本正博、内田郁夫、岩田剛敏、岡本晋、矢部希見子、秋庭正人
    • Organizer
      第96回日本細菌学会関東支部会総会
    • Place of Presentation
      東京ドームホテル
    • Related Report
      2013 Research-status Report
  • [Presentation] Amplification of a multidrug resistance genomic island of Salmonella enterica serovar Typhimurium

    • Author(s)
      秋庭正人
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      タワーホール船堀
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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