Amplification of a novel chromosomal resistance island of Salmonella Typhimurium confers increased resistance to antimicrobials

• Project/Area Number: 25460553
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: AKIBA Masato 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門・細菌・寄生虫研究領域, ユニット長 (60355211)
• Co-Investigator(Renkei-kenkyūsha): KURODA Makoto 国立感染症研究所, 病原体ゲノム解析研究センター, センター長 (80317411)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: Salmonella Typhimurium / 薬剤耐性 / ゲノミックアイランド / 多コピー化 / blaCMY-2

Outline of Final Research Achievements

We found that antimicrobial resistance of Salmonella Typhimurium increased by gene amplification of a novel chromosomal resistance island (GI). Copy number of GI increased up to 85 and the elevation was accompanied by increased amount of transcription. The amplification occurred at the original position. In some cases, the transposition of part of GI occurred after insertion of IS26 or IS1, leading to the amplification of GI at different position. Elevation of transcription of SOS response related genes was observed in a comprehensive gene expression analysis.

Research Products

• [Journal Article] Extensive amplification of GI-VII-6, a multidrug resistance genomic island of Salmonella enterica serovar Typhimurium, increases resistance to extended-spectrum cephalosporins. (2015)
  • Author(s): Lee K, Kusumoto M, Sekizuka T, Kuroda M, Uchida I, Iwata T, Okamoto S, Yabe K, Inaoka T, Akiba M
  • Journal Title: Front Microbiol Volume: 6 Pages: 78-78
  • DOI: 10.3389/fmicb.2015.00078
  • Peer Reviewed / Open Access
• [Journal Article] Whole-Genome Sequence of CMY-2 beta-Lactamase-Producing Salmonella enterica Serovar Typhimurium Strain L-3553 (2014)
  • Author(s): Sekizuka T, Lee K, Kuroda M, Kusumoto M, Iwata T, Uchida I, Tanaka K, Tamamura Y, Akiba M
  • Journal Title: Genome Announc Volume: 2 Issue: 4
  • DOI: 10.1128/genomea.00711-14
  • Peer Reviewed / Open Access
• [Presentation] サルモネラ薬剤耐性アイランド多コピー化の様式解明 (2014)
  • Author(s): 李謙一、関塚剛史、黒田誠、楠本正博、内田郁夫、岩田剛敏、岡本晋、矢部希見子、稲岡隆史、秋庭正人
  • Organizer: 第157回日本獣医学会
  • Place of Presentation: 北海道大学（北海道・札幌市）
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] 多剤耐性Salmonella Typhimuriumのゲノム生物学的特徴 (2014)
  • Author(s): 関塚剛史、李謙一、黒田誠、楠本正博、岩田剛敏、内田郁夫、田中聖、玉村雪乃、秋庭正人
  • Organizer: 第157回日本獣医学会
  • Place of Presentation: 北海道大学（北海道・札幌市）
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] 薬剤耐性アイランドの多コピー化に基づくサルモネラの耐性増強
  • Author(s): 李謙一、稲岡隆史、楠本正博、内田郁夫、岩田剛敏、岡本晋、矢部希見子、秋庭正人
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜大学
• [Presentation] サルモネラ薬剤耐性アイランドの多コピー化に基づく耐性増強
  • Author(s): 李謙一、稲岡隆史、楠本正博、内田郁夫、岩田剛敏、岡本晋、矢部希見子、秋庭正人
  • Organizer: 第96回日本細菌学会関東支部会総会
  • Place of Presentation: 東京ドームホテル
• [Presentation] Amplification of a multidrug resistance genomic island of Salmonella enterica serovar Typhimurium
  • Author(s): 秋庭正人
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: タワーホール船堀