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Development of rapid neutralization assays and a primary transcription assay for human metapneumovirus

Research Project

Project/Area Number 25460563
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Virology
Research InstitutionShiga University of Medical Science

Principal Investigator

Gotoh Bin  滋賀医科大学, 医学部, 教授 (00211920)

Co-Investigator(Renkei-kenkyūsha) KITAGAWA Yoshinori  滋賀医科大学, 医学部, 助教 (00444448)
Research Collaborator YAMAGUCHI Mayu  滋賀医科大学, 大学院医学系研究科, 博士課程・学生
ZHOU Min  滋賀医科大学, 大学院医学系研究科, 博士課程・学生
ITOH Masae  長浜バイオ大学, バイオサイエンス学部, 教授 (10201328)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsヒトメタニューモウイルス / 中和抗体価測定法 / 組換えウイルス / ルシフェラーゼ / 緑色蛍光色素 / 一次転写 / 迅速 / 簡便
Outline of Final Research Achievements

Human metapneumovirus (HMPV) is an important cause of lower respiratory tract infections in young children. Because of its slow replication and weak cytopathic effect in cultured cells, conventional neutralization assays for HMPV require about one week for completion. In this study we established a rapid neutralization assay based on a recombinant virus expressing highly sensitive luciferase genes. This assay could be completed within 12 h and eliminated the requirement of trypsin supporting multistep replication in cultured cells, as well as laborious processes including the plaque assay with immunostaining. Thus we succeeded in establishing the novel neutralization assay for HMPV, which is the fastest and the most straightforward of all previous assays and may be available for high throughput screening of neutralizing antibodies.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (16 results)

All 2015 2014 2013

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 1 results) Presentation (11 results)

  • [Journal Article] Intramolecular complementation of measles virus fusion protein stability confers cell-cell fusion activity at 37 °C.2015

    • Author(s)
      Satoh, Y., Hirose, M., Shogaki, H., Wakimoto, H., Kitagawa, Y., Gotoh, B., Takahashi, K. and Itoh, M.
    • Journal Title

      FEBS Lett.

      Volume: 589 Issue: 1 Pages: 152-158

    • DOI

      10.1016/j.febslet.2014.11.040

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] An anti-interferon activity shared by paramyxovirus C proteins: Inhibition of Toll-like receptor 7/9-dependent alpha interferon induction.2014

    • Author(s)
      Mayu Yamaguchi, Yoshinori Kitagawa, Min Zhou, Masae Itoh, and Bin Gotoh
    • Journal Title

      FEBS letters

      Volume: 588 Issue: 1 Pages: 28-34

    • DOI

      10.1016/j.febslet.2013.11.015

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expeditious neutralization assay for human metapneumovirus based on a recombinant virus expressing Renilla luciferase2013

    • Author(s)
      Min Zhou, Yoshinori Kitagawa, Mayu Yamaguchi, Chika Uchiyama, Masae Itoh, Bin Gotoh.
    • Journal Title

      Journal of Clinical Virology

      Volume: 56 Issue: 1 Pages: 31-36

    • DOI

      10.1016/j.jcv.2012.09.014

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Masae Itoh. F-actin modulates measles virus cell-cell fusion and assembly by altering the interaction between the matrix protein and the cytoplasmic tail of hemagglutinin2013

    • Author(s)
      Hiroshi Wakimoto, Masakatsu Shimodo, Yuto Satoh, Yoshinori Kitagawa, Kaoru Takeuchi, Bin Gotoh
    • Journal Title

      Journal of Virology

      Volume: 87 Issue: 4 Pages: 1974-1984

    • DOI

      10.1128/jvi.02371-12

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Human Parainfluenza Virus Type 2 V Protein Inhibits TRAF6-Mediated Ubiquitination of IRF7 To Prevent TLR7- and TLR9-Dependent Interferon Induction.2013

    • Author(s)
      Kitagawa Y, Yamaguchi M, Zhou M, Nishio M, Itoh M, Gotoh B.
    • Journal Title

      J. Virol.

      Volume: 87 Issue: 14 Pages: 7966-7976

    • DOI

      10.1128/jvi.03525-12

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] NSs protein of SFTS virus targets STAT2 for preventing type I interferon signaling2015

    • Author(s)
      Yoshinori Kitagawa, Madoka Sakai, Masayuki Shimojima, Masayuki Saijyo, Masae Itoh, Bin Gotoh
    • Organizer
      The 63rd annual meeting of the Japanese society for virology
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Sendai virus V protein inhibits the NLRP3 inflammasome activation by preventing the NLRP3 oligomerization2015

    • Author(s)
      Takayuki Komatsu, Yukie Tanaka, Yoshinori Kitagawa, Naoki Koide, Yoshikazu Naiki, Mayu Yamaguchi, Bin Gotoh, Takashi Yokochi
    • Organizer
      The 63rd annual meeting of the Japanese society for virology
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Mechanism how amino acid 465 in the HR-B domain of measles virus fusion protein modulates the fusion activity2015

    • Author(s)
      Hiroko Shogaki, Yuto Satoh, Saeka Yonemori, Mitsuhiro Hirose, Hiroshi Wakimoto, Yoshinori Kitagawa, Bin Gotoh, Ken-ichi Takahashi, Masae Itoh
    • Organizer
      The 63rd annual meeting of the Japanese society for virology
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Nedd4の麻疹ウイルス粒子形成促進作用とそのメカニズム2014

    • Author(s)
      脇本浩史、鈴木翔大、森田健介、佐藤友人、正垣博子、北川善紀、後藤敏、伊藤正恵
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜 会議センター
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Research-status Report
  • [Presentation] SSPEウイルスKobe-1株F蛋白の細胞融合に関わる変異の解析2014

    • Author(s)
      佐藤友人、樋口遙、姜大鵬、西川大智、正垣博子、脇本浩史、北川善紀、後藤敏、伊藤正恵
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜 会議センター
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Research-status Report
  • [Presentation] センダイウイルスCタンパク質によるJAK-STAT経路阻害機構の研究2014

    • Author(s)
      北川善紀、山口まゆ、渡邊摩耶、伊藤正恵、後藤敏
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜 会議センター
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Research-status Report
  • [Presentation] センダイウイルスV蛋白質によるNLRP3 inflammasome活性化の阻害2014

    • Author(s)
      小松孝行、田中幸枝、北川善紀、山口まゆ、小出直樹、内記良一、後藤敏、横地高志
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜 会議センター
    • Year and Date
      2014-11-10 – 2014-11-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 形質細胞様樹状細胞のIFN-α産生とヒトメタニューモウイルス2013

    • Author(s)
      北川善紀、山口まゆ、周敏、伊藤正恵、後藤敏
    • Organizer
      日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒトメタニューモウイルスのpH依存性細胞融合とウイルス侵入様式2013

    • Author(s)
      周敏、北川善紀、山口まゆ、伊藤正恵、後藤敏
    • Organizer
      日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] 麻疹ウイルスF蛋白質のプロテアーゼ感受性決定要因2013

    • Author(s)
      佐藤友人、正垣博子、廣瀬充宏、脇本浩史、高橋健一、北川善紀、竹内薫、後藤敏、伊藤正恵
    • Organizer
      日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] 一酸化窒素産生抑制におけるセンダイウイルスC蛋白質の役割2013

    • Author(s)
      小松孝行、Odkhuu Erdenezaya、竹内健司、小出直樹、横地高志、後藤敏
    • Organizer
      日本ウイルス学会学術集会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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