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Importance of Specification of RNA export pathway for HIV-1 gene expression

Research Project

Project/Area Number 25460564
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Virology
Research InstitutionKyoto University

Principal Investigator

Taniguchi Ichiro  京都大学, ウイルス研究所, 助教 (00467432)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsヒト免疫不全ウイルス / RNA核外輸送 / RNAスプライシング / RNA‐タンパク質複合体 / mRNA前駆体スプライシング
Outline of Final Research Achievements

Although intron-containing HIV-1 RNAs have the same features as cellular general mRNAs, HIV-1 blocks the utilization of mRNA export factor, TAP, during nuclear RNA export. The blockage was achieved by the activity of viral Rev protein to inhibit TAP recruitment to viral RNAs. However, why Rev inhibits the recruitment remains a mystery.
Since viral RNAs are exported without being spliced, Rev may block TAP recruitment to escape the commitment to RNA splicing. I hypothesized that TAP stimulates RNA splicing.
To test the hypothesis, we performed in vitro splicing assay. Splicing products were increased when purified recombinant TAP protein was added. Conversely, RNA splicing was delayed by TAP inhibitor. These results support the hypothesis that TAP stimulates RNA splicing.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2016 2015 2014 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results) Remarks (2 results)

  • [Journal Article] Regulation of proper complex formation for nuclear RNA export2015

    • Author(s)
      谷口一郎、大野睦人
    • Journal Title

      生化学

      Volume: 87 Issue: 1 Pages: 68-74

    • DOI

      10.14952/SEIKAGAKU.2015.870068

    • ISSN
      0037-1017
    • Year and Date
      2015-02-25
    • Related Report
      2014 Research-status Report
  • [Journal Article] Exportin-5 mediates nuclear export of SRP RNA in vertebrates2015

    • Author(s)
      Takeiwa T, Taniguchi I, Ohno M
    • Journal Title

      Genes to Cells

      Volume: 20 Issue: 4 Pages: 281-291

    • DOI

      10.1111/gtc.12218

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment2014

    • Author(s)
      Taniguchi I, Mabuchi N, Ohno M
    • Journal Title

      Nucleic Acids Res

      Volume: 42 Issue: 10 Pages: 6645-58

    • DOI

      10.1093/nar/gku304

    • NAID

      120005458278

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Analysis of RNA transport in Xenopus oocytes and mammalian cells2014

    • Author(s)
      Taniguchi I, McCloskey A, Ohno M
    • Journal Title

      Methods in Cell Biology

      Volume: 122 Pages: 395-413

    • DOI

      10.1016/b978-0-12-417160-2.00018-7

    • Related Report
      2014 Research-status Report
  • [Presentation] Systematic Analysis of hnRNP C Domains in Sorting of RNA Polymerase II Transcripts2016

    • Author(s)
      Sayaka Dantsuji, Ichiro Taniguchi, Mutsuhito Ohno
    • Organizer
      The 14th International Student Seminar
    • Place of Presentation
      京都府 京都市 京都大学 芝蘭会館
    • Year and Date
      2016-03-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitement2015

    • Author(s)
      Ichiro Taniguchi, Naoto Mabuchi, Mutsuhito Ohno
    • Organizer
      The 22nd East Asia Joint Symposium on Biomedical Research
    • Place of Presentation
      沖縄県 沖縄科学技術大学院大学
    • Year and Date
      2015-11-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] HIV-1のRevタンパク質はTAP/NXF1のリクルートを抑制することによりウイルスRNAの核外輸送経路を規定する2014

    • Author(s)
      谷口一郎、馬渕直人、大野睦人
    • Organizer
      第16回日本RNA学会
    • Place of Presentation
      愛知県 名古屋市 ウィンクあいち
    • Year and Date
      2014-07-23 – 2014-07-25
    • Related Report
      2014 Research-status Report
  • [Presentation] HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment2013

    • Author(s)
      Ichiro Taniguchi, Naoto Mabuchi and Mutsuhito Ohno
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Research-status Report
  • [Remarks] 京都大学 ウイルス研究所 情報高分子化学研究分野

    • URL

      http://www.virus.kyoto-u.ac.jp/Lab/ohnolab.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 京都大学ウイルス研究所 情報高分子化学研究分野

    • URL

      http://www.virus.kyoto-u.ac.jp/Lab/ohnolab.html

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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