Establishment of novel anti-virus strategy targeting HIV-1 RNA psi pseudoknot.

• Project/Area Number: 25460567
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: Osaka University
• Principal Investigator: Sakuragi Jun-ichi 大阪大学, 微生物病研究所, 助教 (90273705)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: HIV-1 / RNA / ウイルスゲノム / パッケージング / 二量体化 / HIV / Gag / 感染 / ウイルス / ＨＩＶ / ゲノムパッケージング / 偽結節構造

Outline of Final Research Achievements

The genome of HIV-1 always forms dimer in virion. The genome dimerization is believed to have important roles on viral infectivity and/or pathogenicity. Previously I suggested the dimerization signal region RNA (DLS) of HIV-1 forms pseudoknot-like structure. In this project, I explored a precise functional structures around the pseudoknot, such as SL1 and primer binding site applying virological/molecular biological approach. In addition, I focused on the p1 region, which is important for viral maturation process and examined its roles on viral infectivity and genome packaging.
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Research Products

• [Journal Article] Fibrocytes differ from macrophages but can be infected with HIV-1. (2015)
  • Author(s): M. Hashimoto, H. Nasser, F. Bhuyan, N. Kuse, Y. Satou, S. Harada, K. Yoshimura, J. Sakuragi, K. Monde, Y. Maeda, S. Welbourn, K. Strebel, E.W.A. El-Wahab, M. Miyazaki , S. Hattori, N. Chutiwitoonchai, M. Hiyoshi, S. Oka, M. Takiguchi, S. Suzu.
  • Journal Title: The Journal of Immunology Volume: 195 Issue: 9 Pages: 4341-4350
  • DOI: 10.4049/jimmunol.1500955
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Properties of human immunodeficiency virus type 1 reverse transcriptase recombination upon infection. (2015)
  • Author(s): Sakuragi S, Shioda T, Sakuragi J.
  • Journal Title: J Gen Virol. Volume: 96 Pages: 3382-8
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Efavirenz enhances HIV-1 Gag processing at the plasma membrane through Gag-Pol dimerization (2013)
  • Author(s): Sudo S, Haraguchi H, Hirai Y, Gatanaga H, Sakuragi J-I, Momose F, Morikawa Y
  • Journal Title: J Virol Volume: 87 Issue: 6 Pages: 3348-3360
  • DOI: 10.1128/jvi.02306-12
  • Peer Reviewed
• [Journal Article] HIVの複製プロセス～ウイルスゲノムを中心として～ (2013)
  • Author(s): 櫻木淳一
  • Journal Title: ウイルス Volume: 63 Pages: 175-186
  • NAID: 130004713423
• [Presentation] HIV-1 Gag p1ペプチドのウイルス生活環における機能的解析 (2015)
  • Author(s): 櫻木 淳一
  • Organizer: 第29回日本エイズ学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2015-11-29
• [Presentation] HIV-1 Gag p1ペプチドのウイルス生活環における機能的解析 (2015)
  • Author(s): 櫻木 淳一
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2015-11-22
• [Presentation] HIV-1ゲノムRNA二量体化領域DLSの機能的構造に関する解析 (2015)
  • Author(s): 櫻木 淳一
  • Organizer: 第17回日本RNA学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2015-07-15
• [Presentation] HIVゲノムRNAとその周辺 シンポジウム13「HIVのウイルス学」 (2014)
  • Author(s): 櫻木淳一
  • Organizer: 日本エイズ学会
  • Place of Presentation: 大阪
  • Year and Date: 2014-12-03 – 2014-12-05
  • Invited
• [Presentation] HIV-1パッケージングシグナル内最重要領域SL1の機能的構造に関する多角的解析 (2014)
  • Author(s): 櫻木淳一
  • Organizer: 日本エイズ学会
  • Place of Presentation: 大阪
  • Year and Date: 2014-12-03 – 2014-12-05
• [Presentation] HIV-1パッケージングシグナル内最重要領域SL1の機能的構造に関する多角的解析 (2014)
  • Author(s): 櫻木淳一
  • Organizer: 日本ウイルス学会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-10 – 2014-11-12
• [Presentation] SL1 REVISITED: FUNCTIONAL ANALYSIS OF THE STRUCTURE AND CONFORMATION OF HIV-1 GENOME RNA. (2014)
  • Author(s): Jun-ichi Sakuragi.
  • Organizer: Retrovirus meeting at Cold Spring Harbor Laboratory.
  • Place of Presentation: NY, USA.
  • Year and Date: 2014-05-19 – 2014-05-24
• [Presentation] Relationship between genome packaging and Gag translation of various mammal retroviruses. (2013)
  • Author(s): Sakuragi J.
  • Organizer: 25th Workshop on Retroviral Pathogenesis.
  • Place of Presentation: Reykjavik, Iceland.
• [Presentation] Virology. Training Course (2013)
  • Author(s): Sakuragi J.
  • Organizer: The 12th Awaji International Forum on Infection and Immunity
  • Place of Presentation: Awaji, Japan.
  • Invited
• [Presentation] レンチウイルスのGag開始コドンおよび翻訳とゲノムパッケージングに関する解析 (2013)
  • Author(s): 櫻木淳一・夏井洋和・櫻木小百合・塩田達雄
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
• [Presentation] HIVサブタイプ比較によるゲノムパッケージングに関する解析 (2013)
  • Author(s): 櫻木淳一・櫻木小百合・塩田達雄
  • Organizer: 第27回日本エイズ学会学術集会・総会
  • Place of Presentation: 熊本
• [Presentation] レトロウイルスのGagAUGおよび翻訳とゲノムパッケージングに関する解析 (2013)
  • Author(s): 櫻木淳一・夏井洋和・櫻木小百合・塩田達雄
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸