| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We have analyzed the machanism of genome replication of Aichi virus, a picornavirus associated with acute gastroenteritis in humans. In this study, we identified novel host factors required for Aichi virus genome replication. In addition, we revealed involvement of lipids in genome replication. At replication sites of Aichi virus genome, viral 2B, 2BC, 2C, 3A, and 3AB proteins and multiple host proteins were assembled through protein-protein interactions, resulting in accumulation of two kinds of lipid, PI4P and cholesterol. Moreover, accumulation of these lipids was indicated to be essential for Aichi virus genome replication.
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