| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Previously we identified a few anti-cytomegalovirus (CMV) and anti-varicella zoster virus (VZV) compounds, respectively, by screening of a 9600 chemical compound library with reporter cell lines that can detect each virus quickly. In this study, we analyzed effects of the identified compounds on viral DNA replication and gene expression as well as on viral growth properties, and found the followings: 1) anti-VZV compound 45B5 targets the portal protein encoded by ORF54 and probably inhibits the process of viral DNA injection into the nuclei. 2) anti-VZV and -CMV compound 133G4 inhibits the transactivation functions of immediate-early proteins encoded by VZV, CMV, and herpes simplex virus, probably through a host factor. 3) 35C10 inhibits a process between the penetration of virus into cells and the expression of immediate-early protein.
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