Analysis of replication mechanism and pathogenesis of hepatitis E virus using infectious clone
Project/Area Number |
25460580
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Virology
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Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
ISHII Koji 国立感染症研究所, ウイルス第二部, 室長 (40280763)
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Co-Investigator(Kenkyū-buntansha) |
Li Tian Cheng 国立感染症研究所, ウイルス第二部, 主任研究官 (90370957)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | E型肝炎ウイルス / 複製機構 / 感染メカニズム / 病原性 / レプリコン / プロテアーゼ |
Outline of Final Research Achievements |
We constructed infectious hepatitis E virus (HEV) clone from infected porcine liver and replaced the structural protein to reporter genes resulted in successful construction of HEV replicon. This replicon was used for the screening of the library of chemical compounds. We found some compounds that shared the same bioactivity. Although more detailed analysis is still needed to reveal the effect of these compounds to HEV replication, but these results would contribute to the analysis of the mechanism of HEV replication and pathogenesis. We also analyzed the processing of HEV nonstructural protein (ORF1) that encodes viral proteins responsible for the replication of HEV. We expressed whole ORF1 and potential protease region by using wheat germ-based cell free expression system. Protease activity was detected from ORF1 protein, suggesting that this cell free expression system is useful for the analysis of the processing mechanism of HEV nonstructural protein.
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Report
(4 results)
Research Products
(52 results)
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[Journal Article] A Case of Chronic Hepatitis E Infection in a Persistently Immunosuppressed Patient Unable to be Eliminated after Ribavirin Therapy.2016
Author(s)
Miyoshi M., Kakinuma S., Tanabe Y., Ishii K., Li T.C., Wakita T,, Tsuura Y., Watanabe H., Asahina Y., Watanabe M. and Ikeda T.
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Journal Title
Internal Medicine
Volume: in press
Pages: in press
Related Report
Peer Reviewed
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[Journal Article] Epidemiological and genetic analysis of a 2014 outbreak of hepatitis A in Japan2015
Author(s)
Ishii K., Kiyohara T., Yoshizaki S., Kawabata K., Kanayama A., Yahata Y., Takahashi T., Kinoshita H., Saitou T., Sunagawa T., Oishi K., Uema M., Noda M. and Wakita T.
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Journal Title
Vaccine
Volume: 33
Pages: 6029-6036
DOI
Related Report
Peer Reviewed
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[Journal Article] Epidemiological and genetic analysis of a 2014 outbreak of hepatitis A in Japan.2015
Author(s)
Ishii K., Kiyohara T., Yoshizaki S., Kawabata K., Kanayama A., Yahata Y., Takahashi T., Kinoshita H., Saitou T., Sunagawa T., Oishi K., Uema M., Noda M. and Wakita T.
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Journal Title
Vaccine
Volume: 33
Pages: in press
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular detection of hepatitis E virus in rivers in the Philippines.2014
Author(s)
Li T.C., Yang, T., Shiota T., Yoshizaki S., Yoshida H., Saito M., Imagawa T., Malbas F., Lupisan S., Oshitani H., Wakita T. and Ishii K.
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Journal Title
American Journal of Tropical Medicine and Hygine
Volume: 90
Pages: 764-766
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Chronic Hepatitis B Prevalence among Children and Mothers: Results from a Nationwide, Population-Based Survey in Lao People’s Democratic Republic.2014
Author(s)
Xeuatvongsa A., Komada K., Kitamura T., Vongphrachanh P., Pathammavong C., Phounphenghak K., Sisouk T., Phonekeo D., Sengkeopaseuth B., Som-Oulay V., Ishii K., Wakita T., Sugiyama M. and Hachiya M.
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Journal Title
PLOS One
Volume: 9
Pages: e88829
DOI
Related Report
Peer Reviewed
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[Journal Article] Neutralizing antibodies induced by cell culture-derived hepatitis C virus was effective both in vitro and in vivo.2013
Author(s)
Akazawa D., Moriyama M., Yokokawa H., Omi N., Watanabe N., Date T., Morikawa K., Aizaki H., Ishii K., Kato T., Mochizuki H., Nakamura N. and Wakita T.
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Journal Title
Gastroenterology
Volume: 145
Pages: 447-455
DOI
Related Report
Peer Reviewed
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