| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Follicular dendritic cells (FDCs) play a critical role for germinal center (GC) reactions. We found that a novel class of monocytic cells, named as FDMC, during the analysis of a mouse FDC line, FL-Y. In this study, we examined the mechanisms underlying FDMC differentiation and its immunological functions. As a result, I found that CSF-1R signaling was necessary to FDMC differentiation, which was triggered by IL-34 produced from FL-Y. In immunological functions of FDMC, FDMC significantly enhanced the cell division and the expression of GC-markers on anti-CD40-stimulated B cells in vitro. Furthermore, FDMC induced somatic hypermutation at immunoglobulin variable gene in anti-CD40-stimulated B cells. Finally, I detected FDMC-like cells in the spleen of immunized mice. These results suggest that IL-34 and FDMC might be involved in GC reactions in vivo.
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