Elucidating the activation mechanisms of germinal center B cell induced by monocytic cells depending on follicular dendritic cells.
Project/Area Number |
25460590
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Immunology
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Research Institution | Okayama University |
Principal Investigator |
Magari Masaki 岡山大学, 自然科学研究科, 助教 (50359882)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 濾胞樹状細胞 / 胚中心 / IL-34 / CSF-1 / CSF-1受容体 / 抗体の親和性成熟 / 抗体 / 体細胞高頻度突然変異 / 親和性成熟 |
Outline of Final Research Achievements |
Follicular dendritic cells (FDCs) play a critical role for germinal center (GC) reactions. We found that a novel class of monocytic cells, named as FDMC, during the analysis of a mouse FDC line, FL-Y. In this study, we examined the mechanisms underlying FDMC differentiation and its immunological functions. As a result, I found that CSF-1R signaling was necessary to FDMC differentiation, which was triggered by IL-34 produced from FL-Y. In immunological functions of FDMC, FDMC significantly enhanced the cell division and the expression of GC-markers on anti-CD40-stimulated B cells in vitro. Furthermore, FDMC induced somatic hypermutation at immunoglobulin variable gene in anti-CD40-stimulated B cells. Finally, I detected FDMC-like cells in the spleen of immunized mice. These results suggest that IL-34 and FDMC might be involved in GC reactions in vivo.
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Report
(4 results)
Research Products
(7 results)
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[Presentation] Follicular dendritic cells induce a new class of myeloid cells with B cell stimulating activities: specific dependence on Il-34/CSF-1R signaling pathway.2013
Author(s)
Yamane F., Matsui, K., Tanimoto, H., Iwasaki, E., Asakura, M., Tokumitsu, H., Nishikawa, Y., Kanayama, N., Magari, M., Ohmori, H.
Organizer
15th International Congress of Immunology
Place of Presentation
ミラノ(イタリア)
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