| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Active immunization with amyloid beta peptide (Abeta) induced autoimmune encephalitis in humans, suggesting that Abeta-reactive encephalitogenic T cells exist in the peripheral blood and are involved in chronic inflammation of AD brain. In order to prove this, we tried to establish Abeta-reactive encephalitogenic T cell clones. After immunization of interferon-gamma mice with Abeta and Freund’s complete adjuvant, lymphocytes were obtained from popliteal lymph nodes and the spleen, and repeatedly stimulated with Abeta in the presence of interleukin-12. We could observe significantly higher proliferative responses against Abeta. However, while we are enriching Abeta-reactive T cells in vitro, we had mycoplasma infection in the laboratory, and this project is suspended.
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