| Project/Area Number |
25460601
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUBA Shintaro 金沢医科大学, 医学部, 助教 (40367462)
WADA Toshiki 金沢医科大学, 医学部, 講師 (10451634)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | アレルギー / 免疫学 / セリンプロテアーゼインヒビター / 好塩基球 / 好酸球 / 気管支喘息 |
|---|
| Outline of Final Research Achievements |
Mast cells, basophils and eosinophils secrete abundant serine proteases as well as chemical mediators and cytokines.However, it remains unclear about expression profiles and functions of serine protease inhibitors in allergic effector cells. In this study, we have found that a major serine protease inhibitor, secretory leukoprotease inhibitor (SLPI) is expressed on basophils and eosinophils. Both basophils and eosinophils from SLPI-deficient (Slpi-/-) mice showed more cytokine secretions than those from wild-type (WT) mice upon stimulation. Mice transferred with Slpi-/- basophils showed an enhanced IgE-mediated cutaneous anaphylaxy when compared with WT mice. We also investigated HDM induced asthma model. Slpi-/- mice showed significantly higher percentages of eosinophils in Blonchoalveolar lavage fluids than WT mice. These results suggested that SLPI is essential for the regulation of basophil and eosinophil activation.
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