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Analysis of the regulatory role of SLPI in the activation of basophils and eosinophils.

Research Project

Project/Area Number 25460601
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Immunology
Research InstitutionKanazawa Medical University

Principal Investigator

NAKAMURA Akira  金沢医科大学, 医学部, 教授 (20344723)

Co-Investigator(Renkei-kenkyūsha) MATSUBA Shintaro  金沢医科大学, 医学部, 助教 (40367462)
WADA Toshiki  金沢医科大学, 医学部, 講師 (10451634)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsアレルギー / 免疫学 / セリンプロテアーゼインヒビター / 好塩基球 / 好酸球 / 気管支喘息
Outline of Final Research Achievements

Mast cells, basophils and eosinophils secrete abundant serine proteases as well as chemical mediators and cytokines.However, it remains unclear about expression profiles and functions of serine protease inhibitors in allergic effector cells. In this study, we have found that a major serine protease inhibitor, secretory leukoprotease inhibitor (SLPI) is expressed on basophils and eosinophils. Both basophils and eosinophils from SLPI-deficient (Slpi-/-) mice showed more cytokine secretions than those from wild-type (WT) mice upon stimulation. Mice transferred with Slpi-/- basophils showed an enhanced IgE-mediated cutaneous anaphylaxy when compared with WT mice. We also investigated HDM induced asthma model. Slpi-/- mice showed significantly higher percentages of eosinophils in Blonchoalveolar lavage fluids than WT mice. These results suggested that SLPI is essential for the regulation of basophil and eosinophil activation.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (11 results)

All 2015 2014 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] A critical role of Dectin-1 in hypersensitivity pneumonitis2015

    • Author(s)
      Higashino-Kameda M, Yabe-Wada T, Matsuba S, Takeda K, Anzawa K, Mochizuki T, Makimura K, Saijo S, Iwakura Y, Toga H, Nakamura A
    • Journal Title

      Inflamm Res

      Volume: 65 Issue: 3 Pages: 235

    • DOI

      10.1007/s00011-015-0910-1

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 呼吸器系の宿主免疫応答2015

    • Author(s)
      中村晃
    • Journal Title

      呼吸

      Volume: 34 Pages: 241-246

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Dichotomy in the FcγRIIB deficiency and autoimmune-prone SLAM haplotype clarifies the roles of the Fc receptor in development of autoantibodies and glomerulonephritis.2014

    • Author(s)
      Kanari Y, Sugahara–Tobinai A, Takahashi H, Inui M, Nakamura A, Hirose S, Takai T.
    • Journal Title

      BMC Immunol

      Volume: 15 Issue: 1 Pages: 47-47

    • DOI

      10.1186/s12865-014-0047-y

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Secretory leukocyte protease inhibitor modulates urethane-induced lung carcinogenesis.2014

    • Author(s)
      Jan Treda C, Fukuhara T, Suzuki T, Nakamura A, Zaini J, Kikuchi T, Ebina M, Nukiwa T.
    • Journal Title

      Carcinogenesis

      Volume: 35 Issue: 4 Pages: 896-904

    • DOI

      10.1093/carcin/bgt382

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] SLPI regulates eosinophil-mediated allergic inflammation2015

    • Author(s)
      松葉慎太郎、武田和也、和田俊樹、高井俊行、中村晃
    • Organizer
      第44回日本免疫学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市白石区)
    • Year and Date
      2015-11-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Functional analysis of Sortilin in plasmacytoid dendritic cells2015

    • Author(s)
      和田俊樹、松葉慎太郎、武田和也、 Caroline C. Philpott、中村晃
    • Organizer
      第44回日本免疫学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市白石区)
    • Year and Date
      2015-11-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Trichosporon asahiiによる過敏性肺炎モデルにおけるDectin-1の機能解析2015

    • Author(s)
      東野茉莉、及川卓、中川研、水野史朗、長内和弘、西城忍、槇村浩一、岩倉洋一郎、中村晃、 栂博久
    • Organizer
      第55回日本呼吸器学会学術講演会
    • Place of Presentation
      東京国際フォーラム(東京都千代田区)
    • Year and Date
      2015-04-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 好塩基   球・好酸球におけるSLPIの制御機構の解明.2014

    • Author(s)
      松葉慎太郎,和田俊樹,武田和也,佐藤哲也,須山幹太,高井俊行,中村晃
    • Organizer
      第35回日本炎症・再生医学会
    • Place of Presentation
      万国津梁館・沖縄県名護市
    • Year and Date
      2014-07-02
    • Related Report
      2014 Research-status Report
  • [Presentation] A regulatory role of secretory leukoprotease inhibitor (SLPI) in allergic effector cells.

    • Author(s)
      Shintaro Matsuba, Kazuya Takeda, Toshiki Yabe-Wada, Toshiyuki Takai, Akira Nakamura
    • Organizer
      Annual meeting of the Japanese society for immunology
    • Place of Presentation
      Makuhari Messe, Chiba
    • Related Report
      2013 Research-status Report
  • [Presentation] 好塩基球・好酸球におけるSLPIの制御機構の解明

    • Author(s)
      松葉慎太郎、和田俊樹、武田和也、佐藤哲也、須山幹太、高井俊行、中村 晃
    • Organizer
      日本アレルギー学会秋季学術大会
    • Place of Presentation
      ホテルニューオータニ・東京都
    • Related Report
      2013 Research-status Report
  • [Remarks] 金沢医科大学免疫学

    • URL

      http://www.kanazawa-med.ac.jp/~serol/index.html

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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