| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Themis is a newly identified protein that localizes in cytoplasm and nucleus and is essential for inducing T cell development in the thymus. Here we found that Themis binds the adaptor protein Grb2 in the nuclei of T cells and regulates nuclear protein levels of Grb2 in T cells, and that nuclear Themis-Grb2 interaction is required for T cell development. However, the nuclear localization of Themis is dispensable for cytokine production by peripheral T cells, suggesting that Themis has different functions in the thymus and periphery. Our results also show that two CABIT domains in Themis are both required for T cell development and that each of the CABIT domains exerts distinct functions during T cell development.
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