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Study on super human dried amniotic membrane manufacture technology aiming at the manufacture of new materials for the regenerative therapy

Research Project

Project/Area Number 25460647
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied pharmacology
Research InstitutionUniversity of Toyama

Principal Investigator

Okabe Motonori  富山大学, 大学院医学薬学研究部(医学), 助教 (60283066)

Co-Investigator(Kenkyū-buntansha) 吉田 淑子  富山大学, 大学院医学薬学研究部(医学), 准教授 (00171421)
相古 千加  富山大学, 大学院医学薬学研究部(医学), 助教 (10523889)
二階堂 敏雄  富山大学, 学内共同利用施設等, 理事・副学長 (50180568)
野村 義宏  東京農工大学, 農学部, 教授 (10228372)
林 京子  中部大学, 付置研究所, 研究員 (60110623)
Co-Investigator(Renkei-kenkyūsha) MIZUTA SYOUSHI  福井県立大学, 生物資源学, 准教授 (30254246)
DOKI YOSHINORI  富山大学, 大学院医学薬学研究部(医学), 講師 (90303221)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsヒト組織利用研究 / ハイパードライヒト乾燥羊膜 / 抗生物質 / 抗菌効果 / 定量 / 国際特許 / ウイルス不活化 / ヒト乾燥羊膜
Outline of Final Research Achievements

The human amniotic membrane (AM) has been widely used in ophthalmic surgery. However, it has never been explored that AM is effective for the release of impregnated drugs.
Hyperdry-AM (HD-AM), glutaraldehyde cross-linked HD-AM (Glu HD-AM) and a solvent of poly-N-p-vinylbenzyl-D-lactonamide (PVLA) were all found not to cause irritation to the rabbit eye. Residual glutaraldehyde in Glu HD-AM was about 40 ppm (dry-weight basis). HD-AM (ACV/PVLA HD-AM) loaded Acyclovir (ACV; hydrophobic medicine) containing PVLA-bearing nanospheres (ACV/PVLA) was more effective against a herpes simplex virus-1 (HSV-1) eye infection model and its efficacy was dose- and volume-dependent.
A combination of HD-AM and PVLA can be working as drug delivery system that may be possible to controlle the release of hydrophobic medicines.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report

Research Products

(38 results)

All 2016 2015 2014 2013 Other

All Journal Article (8 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (26 results) (of which Invited: 5 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results) Funded Workshop (1 results)

  • [Journal Article] Use of hyperdry amniotic membrane in operations for cleft palate: a study in rats.2015

    • Author(s)
      Tsuno H, Noguchi M, Okabe M, Tomihara K, Yoshida T, Nikaido T.
    • Journal Title

      British Journal of Oral and Maxillofacial Surgery

      Volume: 53 Pages: 358-363

    • DOI

      10.1016/j.bjoms.2015.01.018

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Bioprinting with pre-cultured cellular constructs towards tissue engineering of hierarchical tissues.2015

    • Author(s)
      Nakamura M, Mir T A, Arai K, Ito S, Yoshida T, Iwanaga S, Kitano H, Obara C, Nikaido T..
    • Journal Title

      International Journal of Bioprinting,

      Volume: 1(1) Pages: 39-48

    • DOI

      10.18063/ijb.2015.01.007

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Human Amnion-derived stem cells have immunosuppressive properties.2015

    • Author(s)
      Li J, Koike C, Sugimoto J, Yoshida T, Okabe M, and Nikaido T
    • Journal Title

      Cell Transplant

      Volume: 24 Pages: 2065-2076

    • DOI

      10.3727/096368914x685230

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 産科婦人科とレドックス2015

    • Author(s)
      相古千加、二階堂敏雄
    • Journal Title

      別冊 医学のあゆみ レドックスUPDATE -ストレス制御の臨床医学・健康科学

      Volume: 別冊 Pages: 249-252

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Characterization of amniotic stem cells.2014

    • Author(s)
      Koike C, Zhou K, Takeda Y, Fathy M, Okabe M, Yoshida T, Nakamura Y, Kato Y, Nikaido T.
    • Journal Title

      Cellular Reprogramming.

      Volume: 16 Pages: 298-305

    • DOI

      10.1089/cell.2013.0090

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Therapeutic potential of amnion epithelial cells for diabetes.2014

    • Author(s)
      Koike C, Okabe M, Yoshida T, and Nikaido T.
    • Journal Title

      Perinatal Stem Cells

      Volume: 23 Pages: 253-257

    • Related Report
      2014 Research-status Report
    • Open Access
  • [Journal Article] Characteristics of human amniotic membrane and Application to regenerative medicine.2014

    • Author(s)
      Nikaido T.
    • Journal Title

      Placenta

      Volume: 35

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 耳科手術におけるHyperdryヒト乾燥羊膜の使用経験2014

    • Author(s)
      將積日出夫, 藤坂実千郎, 髙倉大匡, 坪田雅仁, 金沢祐治, 舘野宏彦, 岡部素典, 吉田淑子, 二階堂敏雄
    • Journal Title

      耳鼻臨床

      Volume: 107 Pages: 173-80

    • NAID

      130003395623

    • Related Report
      2014 Research-status Report
  • [Presentation] 羊膜間葉系幹細胞 (HAMα)が肝硬変モデルマウスに及ぼす影響2016

    • Author(s)
      吉田淑子、岡部素典
    • Organizer
      第121回日本解剖学会総会
    • Place of Presentation
      福島
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] ハイパードライヒト乾燥羊膜の芽胞菌に対する殺菌効果:抗生物質との比較2016

    • Author(s)
      岡部素典、吉田淑子、坂本純子、北島 勲、小池千加、齋藤 滋、二階堂敏雄
    • Organizer
      第15回日本再生医療学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 肝硬変に対するヒト羊膜間葉系幹細胞( HAMα)移植による効果 ーマウス肝硬変モデルを用いてー2016

    • Author(s)
      吉田 淑子、岡部 素典、吉田 聡、周 凱旋、小池 千加、斎藤 滋、二階堂 敏雄
    • Organizer
      第15回日本再生医療学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] dentification of cancer stem cells/cancer initiating cells in endometrial cancer cells.2016

    • Author(s)
      Yi Sun , Toshiko Yoshida , Motonori Okabe , Kaixuan Zhou , Fang Wang , Chika Soko , Sigeru Saito , and Toshio Nikaido
    • Organizer
      第15回日本再生医療学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] Direct reprogramming of immortalized human amniotic epithelial (iHAE) cells towards insulin producing cells2016

    • Author(s)
      Md. Faruk Hasan, Toshiko Yoshida, Motonori Okabe, Kaixuan Zhou, Chika Soko and Toshio Nikaido
    • Organizer
      第15回日本再生医療学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2016-03-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] Direct reprogramming of immortalized human amniotic epithelial (iHAE) cells towards insulin producing cells.2015

    • Author(s)
      Md. Faruk Hasan, Toshiko Yoshida, Motonori Okabe, Kaixuan Zhou, Chika Soko and Toshio Nikaido.
    • Organizer
      平成27年度 富山大学若手研究者等の学術交流・発表会
    • Place of Presentation
      富山
    • Year and Date
      2015-09-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Identification of cancer stem cells/cancer initiating cells in endometrial cancer cells.2015

    • Author(s)
      Yi Sun, Toshiko Yoshida, Motonori Okabe, Kaixuan Zhou, Fang Wang, Chika Soko, Sigeru Saito, and Toshio Nikaido.
    • Organizer
      平成27年度 富山大学若手研究者等の学術交流・発表会
    • Place of Presentation
      富山
    • Year and Date
      2015-09-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Cancer stem cells/cancer initiating cells in cervical cancer cells.2015

    • Author(s)
      Meijie Sang, Toshiko Yoshida, Motonori Okabe, Chika Koike, Kaixuan Zhou, Yi Sun, Fang Wang, Toshio Nikaido.
    • Organizer
      平成27年度 富山大学若手研究者等の学術交流・発表会
    • Place of Presentation
      富山
    • Year and Date
      2015-09-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] 乾燥羊膜と絹由来タンパクによる新規バイオデバイスの開発2015

    • Author(s)
      岡部素典、吉田 淑子、鈴木 拓馬、小池 千加、野村 義宏、齋藤 滋、二階堂 敏雄
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2015-03-19
    • Related Report
      2014 Research-status Report
  • [Presentation] Immunosuppressive activity of amnion-derived cells2015

    • Author(s)
      Jiali Li、Toshiko Yoshida、Chika Koike-Soko、Motonori Okabe、Jun Sugimoto、Toshio Nikaido
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2015-03-19
    • Related Report
      2014 Research-status Report
  • [Presentation] Direct Reprogramming of Immortalized Human Amniotic Epithelial Cells towards insulin producing cells2015

    • Author(s)
      Hasan Faruk、Toshiko Yoshida、Motonori Okabe、Kaixuan Zhou、Chika Soko、Toshio Nikaido
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2015-03-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 肝細胞の極性を制御できる3次元デバイスの作製2015

    • Author(s)
      荒井 健一、吉田 淑子、岡部 素典、小池 千加、二階堂 敏雄、赤池 敏宏、中村 真人
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2015-03-19
    • Related Report
      2014 Research-status Report
  • [Presentation] Identification of Cancer stem cells/cancer initiating cells in Endometrial cells2015

    • Author(s)
      Yi Sun、Toshiko Yoshida、Motonori Okabe、Kaixuan Zhou、Fang Wang、Chika Koike-soko、Shigeru Saito、Toshio Nikaido
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2015-03-19
    • Related Report
      2014 Research-status Report
  • [Presentation] CHT遺伝指導ヒト羊膜細胞の特徴2015

    • Author(s)
      吉田 佳奈美、吉田 淑子、岡部 素典、周 凱旋、相古 千加、二階堂 敏雄
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2015-03-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 再生医学と研究倫理について.2014

    • Author(s)
      二階堂敏雄
    • Organizer
      平成26年度富山県試験研究機関研究員交流集会
    • Place of Presentation
      富山
    • Year and Date
      2014-12-30
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Characteristics of Human Amniotic Membrane and Application to Regenerative Medicine.2014

    • Author(s)
      Nikaido T
    • Organizer
      浙江大学医学院附属第一医院
    • Place of Presentation
      杭州, 中国
    • Year and Date
      2014-12-09
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] ヒト卵巣癌由来CD24(+)SSEA4(+)細胞の特性.2014

    • Author(s)
      吉田淑子, Wang Fang, Zhou Kaixuan, 古市恵津子, 岡部素典, 吉田佳奈美, 齋藤 滋, 二階堂敏雄
    • Organizer
      日本解剖学会第74回中部支部学術集会
    • Place of Presentation
      石川
    • Year and Date
      2014-10-11
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒト羊膜の生物学的特性と臨床応用2014

    • Author(s)
      二階堂敏雄
    • Organizer
      第22回日本胎盤学会・第31回日本絨毛性疾患研究会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-03
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 卵巣癌に存在するCD24(+)SSEA4(+)細胞の特性.2014

    • Author(s)
      吉田淑子, 王 芳, 齋藤 滋, 二階堂敏雄
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒト羊膜の再生医療への応用.2014

    • Author(s)
      二階堂敏雄
    • Organizer
      第3回富山・バーゼル医薬品研究開発シンポジウム
    • Place of Presentation
      富山
    • Year and Date
      2014-08-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 細胞の甦り-細胞や組織は再生可能か?2014

    • Author(s)
      二階堂敏雄
    • Organizer
      富山大学しらゆり会総会
    • Place of Presentation
      富山
    • Year and Date
      2014-08-01
    • Related Report
      2014 Research-status Report
  • [Presentation] CD24+SSEA4+ovarian carcinoma cells exhibit self renewal ability and tumorigenicity.2014

    • Author(s)
      王 芳, 吉田淑子, 岡部素典, 小池千加, 齋藤 滋, 二階堂敏雄
    • Organizer
      第35回日本炎症・再生医学会
    • Place of Presentation
      沖縄
    • Year and Date
      2014-07-01
    • Related Report
      2014 Research-status Report
  • [Presentation] 羊膜(プラセンタ)は生活習慣病,アンチエイジングに有効!2014

    • Author(s)
      吉田淑子
    • Organizer
      第14回日本抗加齢医学学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2014-06-06
    • Related Report
      2014 Research-status Report
  • [Presentation] 羊膜を利用した再生医療はここまで進んでいる2014

    • Author(s)
      岡部素典
    • Organizer
      第14回日本抗加齢医学学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2014-06-06
    • Related Report
      2014 Research-status Report
  • [Presentation] Application of Amniotic Membrane / Amnion-Derived Cells for Regenerative Medicine.2014

    • Author(s)
      Nikaido T
    • Organizer
      河北医科大学
    • Place of Presentation
      石家庄, 中国
    • Year and Date
      2014-06-05
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Application of Amniotic Membrane / Amnion-Derived Cells for Regenerative Medicine.2014

    • Author(s)
      Nikaido T
    • Organizer
      同済大学附属肺科医院
    • Place of Presentation
      上海, 中国
    • Year and Date
      2014-06-05
    • Related Report
      2014 Research-status Report
    • Invited
  • [Remarks] 富山大学大学院医学薬学研究部(医学)再生医学講座

    • URL

      http://www.med.u-toyama.ac.jp/saiseiigaku/index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 富山大学 医学部 再生医学

    • URL

      http://www.med.u-toyama.ac.jp/saiseiigaku/index.html

    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] Dried Amnion and Method for Drying Treatment of Amnion.2013

    • Inventor(s)
      二階堂敏雄、吉田淑子、岡部素典、戸田文香、北川清隆、荒川雅彦
    • Industrial Property Rights Holder
      国立大学法人 富山大学、サクラ精機(株)
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-08-18
    • Acquisition Date
      2015-01-13
    • Related Report
      2014 Research-status Report
    • Overseas
  • [Funded Workshop] The International Forum on MicroManufacturing & Biofabrication'152015

    • Place of Presentation
      富山
    • Year and Date
      2015-05-19
    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2020-03-30  

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